石蒜碱通过下调细胞周期蛋白D1表达抑制SGC-7901胃癌细胞增殖并促进其凋亡  

Lycorine inhibits the proliferation and promotes apoptosis of SGC-7901 gastric cancer cells by downregulating expression of cyclin D1

作　　者：陈肖 龙潇鸿[2] 廖渝 郑姣妮 CHEN Xiao;LONG Xiaohong;LIAO Yu;ZHENG Jiaoni(Department of Pharmacy,Fourth People＇s Hospital of Chongqing,Chongqing 400014;Department of Pharmacy,Second Affiliated Hospital of Chongqing Medical University,Chongqing 400010;Department of Pharmacy,the Second Affiliated Hospital,Army Medical University,Chongqing 400037,China)

机构地区：[1]重庆市第四人民医院药剂科,重庆400014 [2]重庆医科大学附属第二医院药学部,重庆400010 [3]陆军军医大学第二附属医院药学部,重庆400037

出　　处：《细胞与分子免疫学杂志》2018年第4期354-358,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：重庆市重大科技专项[渝科发计字(2004)27号];重庆市自然科学基金(CSTC2005BB5227)

摘　　要：目的探讨石蒜碱对胃癌SGC-7901细胞增殖及凋亡的影响。方法 CCK-8法检测(0、2、4、8)μmol/L石蒜碱对SGC-7901细胞增殖的影响,确定石蒜碱的半数抑制浓度(IC50)。以IC50的石蒜碱处理SGC-7901细胞48 h,流式细胞术检测细胞周期和细胞凋亡,实时荧光定量PCR检测细胞周期蛋白D1(cyclin D1)mRNA水平,Western blot法检测cyclin D1蛋白水平。结果石蒜碱能够明显抑制胃癌SGC-7901细胞增殖,且呈浓度、时间依赖关系。石蒜碱处理24、48 h,对胃癌细胞的IC50分别为(20.85±2.36)μmol/L和(13.29±1.28)μmol/L。采用13μmol/L石蒜碱处理SGC-7901细胞48 h,可将其细胞周期阻滞在G0/G1期并促进细胞凋亡,且细胞cyclin D1 mRNA和蛋白水平明显降低。结论石蒜碱能够抑制SGC-7901胃癌细胞增殖,诱导细胞周期阻滞在G0/G1期并促进细胞凋亡,其作用机制可能与下调cyclin D1表达有关。Objective To investigate the effect of lycorine on the proliferation and apoptosis of gastric cancer SGC-7901 cells. Methods Effect of lycorine on the proliferation of SGC-7901 cel s and 50% inhibitory concentration（ IC50） of lycorine were determined by CCK-8 assay. SGC-7901 cells were treated with IC50 lycorine,and 48 hours later,cel cycle and apoptosis were detected by flow cytometry,and the level of cyclin D1 mRNA and protein was detected by real-time fluorescence quantitative PCR and Western blot analysis. Results Lycorine obviously inhibited the proliferation of SGC-7901 gastric cancer cells in a concentration-or time-dependent manner. The IC50 of 24 hours and 48 hours to gastric cancer cells treated with lycorine was（ 20. 85 ± 2. 36） μmol/L and（ 13. 29 ± 1. 28） μmol/L,respectively. The cell cycle was arrested in G0/G1 phase,the apoptosis rate of cells increased,and the level of cyclin D1 mRNA and protein decreased significantly 48 hours after the treatment with13 μmol/L lycorine. Conclusion Lycorine can inhibit the proliferation of SGC-7901 gastric cancer cel s,induce cel cycle arrest in G0/G1 phase and promote cel apoptosis. The mechanism may be related to the down-regulation of cyclin D1 expression.

关 键 词：石蒜碱 胃癌 细胞增殖 细胞凋亡 细胞周期蛋白D1(cyclin D1) 

分 类 号：R285.5[医药卫生—中药学] Q279[医药卫生—中医学]