白藜芦醇增加体外培养人卵泡颗粒细胞ATP的生成并促进其增殖  

Resveratrol promotes ATP production and the proliferation of human granulosa cells in vitro

作　　者：李小双[1,2] 刘一凡 陈蕾[1,2] LI Xiaoshuang;LIU Yifan;CHEN Lei(Navy Clinical College,Anhui Medical University,Beijing 100048;Department of Obstetrics and Gynecology,PLA Navy General Hospital,Beijing 100048;Department of Obstetrics and Gynecology,Chinese PLA General Hospital,Chinese PLA Medical School,Beijing 100853,China)

机构地区：[1]安徽医科大学海军临床学院,北京100048 [2]中国人民解放军海军总医院妇产科,北京100048 [3]中国人民解放军总医院妇产科,北京100853

出　　处：《细胞与分子免疫学杂志》2018年第4期359-363,共5页Chinese Journal of Cellular and Molecular Immunology

摘　　要：目的探讨白藜芦醇(Res)对体外培养的人卵泡颗粒细胞的增殖及ATP生成的影响。方法收集不孕症患者的卵泡液,进行卵泡颗粒细胞分离、培养,经纯化和卵泡刺激素受体(FSHR)免疫组织化学染色鉴定后体外培养。实验分为空白对照组、30μmol/L二甲基亚砜(DMSO)溶剂对照组、(10、20、30)μmol/L Res处理组。处理24、48 h,采用CCK-8法检测细胞增殖活性、增强型ATP检测试剂盒检测10μmol/L Res处理组和空白对照组24 h后的ATP生成量。结果分离培养的颗粒细胞特异性表达FSHR,阳性物质位于胞质中,阳性细胞90%以上。人卵泡颗粒细胞体外培养24~72 h,进入对数生长期。与对照组比较,(10、20、30)μmol/L Res处理24 h均可增加人卵泡颗粒细胞增殖活性。10μmol/L Res处理细胞的ATP生成量较对照组明显增加。结论 Res可以促进人卵泡颗粒细胞的增殖活性及ATP的生成。Objective To explore the effects of resveratrol（ Res） on the proliferation and ATP production of human ovarian granulosa cells in vitro. Methods Human follicular granulosa cells obtained from the follicle fluid of infertile patients were purified and identified by immunocytochemistry for follicle stimulating hormone receptor（ FSHR）,and then cultured with medium in vitro. The cells were divided into a blank control group,a solvent control group（ DMSO 30 μmol/L）,and Res-treated group（ 10,20 and 30 μmol/L）. Cell viability was detected by CCK-8 assay. Cellular ATP levels were measured using the ATP assay kit after 24 hours in Res-treated group（ 10 μmol/L） and control group. Results FSHR was immunoreactivepositive in cytoplasm of 90% isolated cells. The proliferation of granulosa cells reached the peak during 24 to 72 hours.Compared with the control group,the proliferation activity of the human follicular granulosa cells treated with Res（ 10,20 and30 μmol/L） for 24 hours significantly increased. The level of ATP in the Res-treated group（ 10 μmol/L） increased compared with the control group after treatment with Res for 24 hours. Conclusion Resveratrol promotes the proliferation activity and increases the ATP production of human granulosa cells in vitro.

关 键 词：卵泡颗粒细胞 白藜芦醇 细胞增殖 

分 类 号：R392.33[医药卫生—免疫学] R285.5[医药卫生—基础医学]