细叶结缕草褐斑病病原菌的分离、鉴定及杀菌剂室内毒力测定  

Isolate and identify the pathogen of brown patch on Zoysia tenuifolia and toxicity test of fungicides against the pathogen

作　　者：章武 牛学礼 刘金祥 黄俊文 林金梅 黎宇婷 ZHANG Wu;NIU Xue-li;LIU Jin-xiang;HUANG Jun-wen;LIN Jin-mei;LI Yu-ting(Lingnan Normal University,Zhanjiang 524048,China;School of Life Science,South ChinaNormal University,Guangzhou 510631,China)

机构地区：[1]岭南师范学院,广东湛江524048 [2]华南师范大学生命科学学院,广东广州510631

出　　处：《草原与草坪》2018年第4期60-66,共7页Grassland and Turf

基　　金：国家自然科学基金(31602002);国家级大学生创新训练计划(201810579587);岭南师范学院博士启动项目(ZL1610);岭南师范学院校级培育项目(LP1861)资助

摘　　要：于2014~2016年,对海南省和广东省由细叶结缕草(Zoy siatenuifolia)建植的多个草坪绿地进行病害调查,发现一种引起块状分布的枯死病害对该草的观赏和使用价值产生严重危害。对各地病害样品取样,并采用植物病理常规组织分离法对病原菌进行分离,根据病原菌培养性状、形态学特征、致病性测定和分子生物学特征(r DNA-ITS序列)鉴定出引起细叶结缕草褐斑病的病原菌为立枯丝核菌(Rhizoctonia solani)。室内测定9种杀菌剂对病原菌毒力的试验,结果表明:5%烯唑醇对R.solani毒力最强,其EC50值为0.055 mg/L;毒力最低的为70%甲基托布津,其EC50值为7.021 mg/L。Field surveys were carried out from 2014 to 2016 in several green lawns of Hainan and Guangdong Province of China. A damage disease which seems like brown patch could affect Zoysia tenuifolia was observed and it has serious influence on the ornament and use value of the grass. The normal tissue isolation method of plant pathology was used to isolate the pathogen. Based on comprehensive analyses cultural observations,morphological characteristics,pathogenicity test and its internal transcribed spacer（ ITS） r DNA region nucleotide sequence,the pathogen causing brown patch was identified as Rhizoctonia solani. The toxicity of 9 fungicides to the pathogen was tested in laboratory,the results show that5% diniconazole had the highest inhibitory effects on Rhizoctonia solani with EC50 at 0. 055 mg/L,whereas 70% thiophanate-methyl had lowest inhibitory effects on R. solani with 7. 021 mg/L. The results provide the method for identify the pathogens and may help exploring management strategies.

关 键 词：细叶结缕草 褐斑病 立枯丝核菌 杀菌剂 

分 类 号：S688.4[农业科学—观赏园艺]