活血降糖饮对肥胖大鼠摄食行为的调节作用及海马CD36表达的影响  

Effect of Huoxue Jiangtang Decoction on Food Preference and CD36 Expression of Hippocampus in Obese Rats

作　　者：刘德亮[1] 渠昕[1] 李惠林[1] 赵恒侠[1] 楚淑芳[1] 庄伟坤 张学文[3] LIU Deliang;QU Xin;LI Huilin;Zhao Hengxia;Chu Shufang;Zhuang Weikun;Zhang Xuewen(Shenzhen Traditional Chinese Medicine Hospital,Shenzhen 518033,China;Fourth Clinical Medicine Hospital of Guangzhou University ofTraditional Chinese Medicine,Shenzhen 518033,China;Shanxi University of Traditional Chinese Medicine,Xianyang 712046,China)

机构地区：[1]深圳市中医院,广东深圳518033 [2]广州中医药大学第四临床医学院,广东深圳518033 [3]陕西中医药大学,陕西咸阳712046

出　　处：《中西医结合研究》2018年第1期22-25,28,共5页Research of Integrated Traditional Chinese and Western Medicine

基　　金：深圳市科技创新委员会基础研究项目(No.JCYJ20170307154753286);深圳市医疗卫生三名工程(No.SZSM201512043)

摘　　要：目的研究活血降糖饮对肥胖和胰岛素抵抗大鼠摄取膳食脂肪偏好的影响并探讨可能的机制。方法通过高脂高热卡饮食诱导肥胖和胰岛素抵抗大鼠模型,将模型大鼠分为模型组、中药组、二甲双胍组,另设正常对照组。药物干预后检测血脂、血糖及血清胰岛素,并通过双瓶喜好实验检测大鼠对膳食脂肪的偏好,通过免疫印迹方法检测大鼠海马组织CD36蛋白表达。结果与模型组比较,各治疗组大鼠体重、血脂、血糖、胰岛素抵抗指数(HOMA-IR)均明显改善,对膳食脂肪的偏好明显降低,海马组织CD36蛋白表达明显下调。结论活血降糖饮的干预可降低肥胖和胰岛素抵抗大鼠在摄食行为中对膳食脂肪的偏好,其作用机制可能与下调海马组织CD36蛋白表达有关。Objective To investigate the effect and possible mechanism of Huoxue Jiangtang decoction on food preference of obese and insulin resistant rats. Methods Obese and insulin resistant rats model were induced by high fat diet.After that,model rats were divided into model group,herb group,and metformin group.Besides,a control group was set.After respective treatment,blood glucose,lipids,and insulin were tested.Preference for fat of rats were examined by two-bottle test and the expression of CD36 protein in hippocampus were tested by western blot. Results In contrast to model group,weight,blood glucose level,blood lipid level,and HOMA-IR were decreased in treatment groups.Meanwhile,preference for fat and the expression of hippocampus CD36 protein in treatment groups were also reduced when compared with model group. Conclusion Huoxue Jiangtang decoction was effective in ameliorating preference for fat in obese and insulin resistant rats,and the mechanisms may be related to reducing the expression of CD36 protein in hippocampus.

关 键 词：活血降糖饮 饮食偏好 CD36 

分 类 号：R285.5[医药卫生—中药学]