人C组轮状病毒VP8^＊蛋白与组织血型抗原的结合特征  

Characterization of binding patterns between VP8^＊ protein of human group C rotavirus and histo-blood group antigens

作　　者：王莉鸿 孙晓曼[2] 李丹地[2] 靳淼[2] 温红玲 段招军[2] Wang Lihong;Sun Xiaoman;Li Dandi;Jin Miao;Wen Hongling;Duan Zhaojun(Department of Virology,School of Public Health,Shandong University,Jinan 250012,China;National Institute for Viral Disease Control and Prevention,Chinese Center for Diseases Control and Prevention.)

机构地区：[1]山东大学公共卫生学院病毒学研究室,济南250012 [2]中国疾病预防控制中心病毒病预防控制所

出　　处：《国际病毒学杂志》2018年第4期220-223,共4页International Journal of Virology

基　　金：国家自然科学基金（81472003,31500139,81601813）

摘　　要：目的 研究人C组轮状病毒SZ272 VP8＊蛋白与组织血型抗原的结合特征.方法 利用原核表达系统表达并纯化人C组轮状病毒SZ272的VP8＊蛋白,通过唾液结合实验分析SZ272 GST-VP8＊融合蛋白与A、AB、B、O和O-（非分泌）型唾液的相互作用;通过寡糖结合实验分析SZ272 GST-VP8＊融合蛋白与不同的组织血型抗原（histo-blood group antigens,HBGAs）寡糖的结合情况.结果 电泳结果显示,有GST-VP8＊融合蛋白目的条带,约为52×103 Da,与预期大小相符.唾液结合实验显示,SZ272 GST-VP8＊融合蛋白与A和AB型唾液结合,而与B、O及非分泌（O-）型唾液不结合;寡糖结合实验显示,SZ272 GST-VP8＊融合蛋白特异性地与A型HBGAs相结合.结论 A型组织血型抗原可能是人C组轮状病毒的潜在受体,为进一步研究C组轮状病毒的感染机制提供了基础和依据.Objective This study was to explore the glycan binding specificity between VP8＊ protein of human group C rotavirus （RVC） strain SZ772 and the histo-blood group antigens （HBGAs）.Methods The VP8＊ protein of human RVC SZ272 was expressed and purified.The interactions between SZ272 GST-VP8＊ fusion protein and different types of saliva samples were characterized by the saliva binding assay.The oligosaccharide binding assay was used to analyze the relationship between SZ272 GST-VP8＊ fusion protein and the HBGAs.Results The GST-VP8＊ fusion protein was expressed and the protein showed one band at about 52×103 Da in the SDS-PAGE as expected.Human RVC SZ272 GST-VP8＊ fusion protein bound type A and AB saliva,and showed no binding effects with type B,O and O saliva.Oligosaccharide binding assay indicated that human RVC SZ272 GST-VP8＊ fusion protein specifically recognized type A glycan.Conclusions Type A histo-blood group antigen may be a potential receptor for the human group C rotavirus SZ272.This provides the basis and evidence for illustrating the mechanism of RVC infection and epidemiology.

关 键 词：轮状病毒属 血型抗原 病毒融合蛋白质类 唾液结合 寡糖结合 

分 类 号：R373[医药卫生—病原生物学]