2008-2017年鄂西北地区脑膜炎奈瑟菌药物敏感性及分子分型研究  

Antibiotic susceptibility and molecular type of Neisseria meningitidis in northwestern Hubei

作　　者：高景枝[1] 张雅婷 何飞 郑向梅[1] 吕静[2] 杨红梅[2] 李国明[2] Gao Jingzhi;Zhang Yating;He Fei;Zheng Xiangmei;Lyu Jing;Yang Hongmei;Li Guoming(Shiyan Center for Disease Control and Prevention,Shiyan 442000,Hubei,China;Hubei Provincial Center for Disease Control and Prevention,Wuhan 430079,Hubei,China)

机构地区：[1]十堰市疾病预防控制中心监测检验科,湖北十堰442000 [2]湖北省疾病预防控制中心卫生检验检测研究所,湖北武汉430079

出　　处：《疾病监测》2018年第7期552-558,共7页Disease Surveillance

基　　金：湖北省十堰市科学技术局项目(No.17Y69);湖北省卫计委科研项目(No.JX6B23)~~

摘　　要：目的分析鄂西北地区2008–2017年分离到的72株脑膜炎奈瑟菌(Nm)的药物敏感性及分子分型特征。方法对Nm菌株进行生化鉴定和血清学分群,并采用荧光聚合酶链式反应进行基因分群,用K-B纸片和E-test检测试纸条进行药敏试验;选取部分菌株进行多位点序列分型(MLST)和脉冲场凝胶电泳(PFGE)。结果血清分群结果为B群14株、C群10株、W群3株、E群1株、不可分群44株。基因分群结果:B群33株、C群10株、W群3株、E群1株,其他及不可分群25株。70株Nm菌株对复方新诺明、萘啶酸、环丙沙星、米诺环素、氯霉素5种抗生素的敏感率分别为7.14%、21.43%、28.57%、98.57%和98.57%,对美罗培南、亚胺培南、阿奇霉素、头孢曲松、头孢噻肟、青霉素和利福平均敏感。17株菌株共有12种序列型别,其中12株菌株不能归入现有克隆群,其他5株属于CC4821(4株)和CC175(1株)。55株菌株分为48种不同的PFGE带型,带型相同的菌株其基因群亦相同。结论 2008–2017年鄂西北地区分离的Nm菌株主要为B群;对复方新诺明、萘啶酸和环丙沙星具有较高的耐药性。MLST分型以CC4821为优势克隆。PFGE分型特征呈多态性,未出现优势带型。Objective To analyze the antibiotic susceptibility and molecular type of 72 Neisseria meningitidis strains isolated in the northwestern area of Hubei province from 2008 to 2017. Methods Biochemical identification and serologically grouping of the isolates were conducted, and genotyping of the isolates was performed by using fluorescent PCR.The K-B and E-test was used to test the drug-susceptibility of the isolates, Multi locus sequence typing（MLST） and pulsed field gel electrophoresis（PFGE） was performed to understand the molecular characteristics of the isolates. Results Among the 72 N. meningitidis strains, 14 belonged to serogroup B, 10 belonged to serogroup C, 3 belonged to serogroup W, 1 belonged to serogroup E and 44 were unspecified. The 72 strains were divided into genogroup B（n=33）, genogroup C（n=10）, genogroup W（n=3）, genogroup E（n=1） and 25 isolates were unspecified. The sensitive rates of the 70 N. meningitidis strains to cotrimoxazole, nalidixic acid, ciprofloxacin, minocycline and chloramphenicol were 7.14%, 21.43%, 28.57%, and 98.57% and98.57%, respectively. The strains were all sensitive to remaining 7 antibiotics, i.e. meropenem, imipenem, azithromycin,ceftriaxone, cefotaxime, penicillin and rifampicin. The results of MLST showed that there were 12 different sequence types（STs） in 17 strains, 12 of the strains were unclassified complexes, the other 5 strains belonged to CC4821（n=4） and CC175（n=1）. The PFGE showed that 55 N. meningitidis strains could be classified into 48 different PFGE types, the strains with same PFGE type had same genotype. Conclusion N. meningitidis B strain was predominant in northwestern Hubei from 2008 to 2017. The strain was highly resistant to cotrimoxazole, nalidixic acid and ciprofloxacin. MLST results showed that CC4821 was the predominant clonal complex. PFGE results showed the polymorphism, and no predominant PFGE pattern was found.

关 键 词：脑膜炎奈瑟菌 荧光聚合酶链式反应 药敏试验 脉冲场凝胶电泳 多位点序列分型 

分 类 号：R512.3[医药卫生—内科学]