基质辅助激光解吸/电离质谱质量分析器技术综述  

Advance in Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

作　　者：张国辉 孙传强[1] 孙运[1] 赵学玒[2] 汪曣[1,2] Zhang Guohui;Sun Chuanqiang;Sun Yun;Zhao Xuehong;Wang Yan(School of precision Instrument and Optoelectronics Engineering,Tianjin University,Tianjin 300072,China;Tianjin Key laboratory of Biomedical Dectection Technology and Instrument,Tianjin University,Tianjin 300072,China)

机构地区：[1]天津大学精密仪器与光电子工程学院,天津300072 [2]天津大学天津市生物医学检测技术与仪器重点实验室,天津300072

出　　处：《真空科学与技术学报》2018年第8期667-676,共10页Chinese Journal of Vacuum Science and Technology

基　　金：国家高技术研究发展计划资助项目(863计划);"激光解吸基体辅助离子源-蛋白测序仪器"(No.2014AA020901)

摘　　要：基质辅助激光解吸/电离质谱(MALDI-MS)作为一种软电离质谱分析技术,具有灵敏度高、操作简单、适用范围广等特点,可以用于常规离子化方法较难电离样品分析,目前已被广泛应用于蛋白质、多肽、低聚核苷酸、低聚糖、合成聚合物等的分析。近年来MALDI-MS仪器技术有了较大进展,本文主要从MALDI-MS的基本原理、仪器技术研究进展方面进行了综述,对基质辅助激光解吸/电离离子源与多种质量分析器的串联研究进行了讨论。The latest development in matrix-assisted laser desorption/ionization mass spectrometry（ MALDIMS）,a soft ionization mass spectrometry,were tentatively reviewed. The discussions,with specific examples reported in literature,focused on： i） the operation principle and technical strengths,including the high sensitivity,easy operation and wide application; ii） the progress made in instrumentation and methodologies; iii） the increasingly wide applications in biochemistry,organic chemistry,chemical and pharmaceutical industries,including but not limited to the characterization of proteins,peptides,oligonucleotides,oligosaccharides,and synthetic polymers; and iv） the possible combination of MALDI ion source with a variety of mass analyzers. In addition,the development trends,existing technical problems and possible solutions were also briefly discussed in a thought-provoking way.

关 键 词：基质辅助激光解吸/电离质谱 软电离 生物大分子 质量分析器 串联质谱 

分 类 号：TH843[机械工程—仪器科学与技术]