E-选择素G98T位点基因多态性与乙肝HBV-DNA载量的相关性研究  

Study on correlation between E-selection G98T polymorphism and HBV-DNA load

作　　者：郭清江[1] Guo Qingjiang(Department of Laboratory,the First People＇s Hospital of Bengbu,Bengbu,Anhui,233000,China)

机构地区：[1]蚌埠市第一人民医院检验科,安徽蚌埠233000

出　　处：《西南国防医药》2018年第9期848-851,共4页Medical Journal of National Defending Forces in Southwest China

摘　　要：目的研究E-选择素G98T位点基因多态性与乙肝HBV-DNA载量的相关性。方法选取本院收治的80例乙肝患者作为观察组,另选同期于本院体检的80例健康体检者作为对照组,抽取空腹静脉血5 ml,利用聚合酶链反应-限制性片段长度多态性技术检测E-选择素G98T位点基因多态性,实时荧光定量聚合酶链反应检测HBV-DNA载量。结果两组E-选择素G98T位点基因型与基因频率相比较,观察组AA基因型占比、A等位基因频率低于对照组,AC基因型占比、C等位基因频率高于对照组(P <0.05);观察组内AA基因型患者HBV-DNA载量低于AC基因型患者(P <0.05);依据HBV-DNA正常上限值(1×10~3copy/ml),<1×10~3copy/ml者AA基因型占比及A等位基因频率高于≥1×10~3 copy/ml者,AC基因型占比、C等位基因频率低于≥1×10~3copy/ml者(P <0.05)。结论 E-选择素G98T位点基因存在多态性,AC位点多态性可能增强HBVDNA复制能力。Objective To study the correlation between E-selection G98T polymorpohism and HBV-DNA load. Methods A batch of 80 hepatitis B patients admitted to our hospital were selected to constitute an observation group, and another 80 healthy subjects who had received physical examination in our hospital during the corresponding period were selected to constitute a control group. From eveey patient, 5 ml fasting venous blood was drawn. Polymerase chain reaction-restriction fragment length polymorpohism （PCR-RFLP） was adopted to detect E-selection G98T polymorpohism. Real-time fluorescence quantitative PCR was used to detect HBV-DNA load. Results The genotype and frequency of E-selection G98T loci were compared between the two groups. The proportion of AA genotype and A allele frequency in the observation group were lower than those in the control group, while the proportion of AC genotype and C allele frequency in the observation group were lower than those in the control group （P 〈 0.05）; according to the normal upper limit of HBV-DNA （1×10^3 copy/ml）, the proportion of AA genotype and A allele frequency in subjects with a HBV-DNA load 〈1×10^3 copy/ml were higher than those in subjects with a HBV-DNA load ≥ 1×10^3 copy/ml, while the proportion of AC genotype and C allele frequency were lower than those in subjects with a HBV-DNA load ≥1×10^3 copy/m （P 〈 0.05）. Conclusion E-selection Gene G98T has polymorphism. AC polymorphism may enhance the replication ability of HBV-DNA.

关 键 词：E-选择素 G98T位点 基因多态性 乙型肝炎 HBV-DNA载量 

分 类 号：R512.62[医药卫生—内科学]