曲美他嗪对重症肺炎大鼠保护作用的机制研究  

Protective mechanism of trimetazidine on rats with severe pneumonia

作　　者：王海滨[1] 彭雪[1] 李英[1] WANG Hai-bin;PENG Xue;LI Ying(Griatric Department,Cangzhou Central Hospital of Hebei,Cangzhou,Hebei 061000,China)

机构地区：[1]沧州市中心医院老年内科,河北沧州061000

出　　处：《临床肺科杂志》2018年第10期1829-1833,共5页Journal of Clinical Pulmonary Medicine

摘　　要：目的探讨曲美他嗪对重症肺炎大鼠的保护作用。方法将大鼠气管注入肺炎克雷伯菌液,建立重症肺炎大鼠模型,大鼠被分为对照组、模型组和曲美他嗪组,感染的第7、9、11天,参照白细胞介素-6(IL-6)、白细胞介素-8(IL-8)的ELISA试剂盒说明检测血清中IL-6、IL-8的含量;根据吸光度变化计算肺组织髓过氧化物酶(MPO)活性;Western bloting检测感染的第11天肺组织基质金属蛋白酶-2(MMP-2)和基质金属蛋白酶-9(MMP-9)及NF-κB P65的蛋白表达。结果模型组、曲美他嗪组在第7、9、11天IL-6、IL-8含量及MPO活性均显著高于对照组(P<0.05),而曲美他嗪组在第7天IL-6、IL-8含量与模型组差异无统计学意义(P>0.05),第9天和11天IL-6、IL-8含量均显著低于模型组(P<0.05),三个时间点MPO活性均显著低于模型组(P<0.05);模型组、曲美他嗪组MMP-2、MMP-9、NF-κB P65蛋白表达均显著高于对照组(P<0.05),而曲美他嗪组MMP-2、MMP-9、NF-κB P65蛋白表达均显著低于模型组(P<0.05)。结论曲美他嗪可通过降低炎症因子IL-6和IL-8含量,抑制MPO活性,下调MMP-2和MMP-9及NF-κB信号通路NF-κB P65蛋白表达,从而对重症肺炎大鼠起保护作用。Objective To investigate the protective effect of trimetazidine on severe pneumonia in rats.Methods A rat model of severe pneumonia was established by injecting Klebsiella pneumoniae into the trachea of rats,and then they were divided into the control group,the model group and the trimetazidine group.At the seventh,ninth,and eleventh days after infection,the contents of IL-6 and IL-8 in serum were detected by IL-6 and IL-8 ＇s ELISA kit.The activity of myeloperoxidase（ MPO） in lung tissue was calculated according to the change of absorbance.The expression of MMP-2,MMP-9 and NF-κB P65 protein in lung tissues at the eleventh day after infection were detected by Western blotting.Results The levels of IL-6,IL-8 and MPO activity in the model group and the trimetazidine group were significantly higher than those in the control group at the seventh,ninth and eleventh days（P〈0.05）.The content of IL-6 and IL-8 in the trimetazidine group was not significantly different from the model group at the seventh day（ P〉0.05）.The contents of IL-6 and IL-8 at the ninth and eleventh days were significantly lower than those in the model group（ P〈0.05）,and the activity of MPO at the three time points was significantly lower than that in the model group（ P〈0.05）.The expressions of MMP-2,MMP-9 and NF-κB P65 in the model group and the trimetazidine group were significantly higher than those in the control group,while the expression of MMP-2,MMP-9 and NF-κB P65 in the trimetazidine group was significantly lower than that in the model group（ P〈0.05）.Conclusion Trimetazidine can protect the rats with severe pneumonia by reducing the content of inflammatory factors IL-6 and IL-8,inhibiting the activity of MPO and down regulating the expressions of MMP-2,MMP-9 and NF-κB signaling pathway NF-κB P65 protein.

关 键 词：曲美他嗪 重症肺炎大鼠 NF-ΚB途径 

分 类 号：R563.1[医药卫生—呼吸系统]