真菌NBERC＿170302中次生代谢产物Viridicatumtoxin A的分离纯化及农药活性  

Isolation,Purification and Pesticide Activity of the Secondary Metabolite Viridicatumtoxin A from Fungi NBERC＿170302

作　　者：吴兆圆 张亚妮[1] 王燕 方伟[1] 张志刚[1] WU Zhao-yuan;ZHANG Ya-ni;WANG Yan;FANG Wei;ZHANG Zhi-gang(Hubei Academy of Agricultural Sciences/Hubei Center for Biopesticide Engineering,Wuhan 430064,China)

机构地区：[1]湖北省农业科学院/湖北省生物农药工程研究中心,武汉430064

出　　处：《湖北农业科学》2018年第17期56-58,69,共4页Hubei Agricultural Sciences

基　　金：国家重点研发计划项目(2017YFD0201301-4);湖北省技术创新专项重大项目(2017ABA160);湖北省农业科技创新中心(2016-620-000-001-039)

摘　　要：真菌次生代谢产物一直以来都是药物及其先导化合物的重要来源。在真菌源农药活性天然产物的筛选过程中,得到1株具有杀菌和杀虫活性的真菌NBERC_170302,通过活性跟踪以及菌株大量发酵,经提取和制备高效液相色谱分离纯化得到其中的活性化合物,并通过X-射线单晶衍射鉴定为Viridicatumtoxin A,进一步农药活性测试表明,对多种农业致病真菌具有抑制作用,其中对于小麦赤霉菌、灰霉菌和小麦颖枯病菌具有明显活性,并具有中等杀小菜蛾的活性。Secondary metabolites from fungal have always been an important source of drugs and leading compounds. In the search for pesticides of natural products from fungus, fungus NBERC_170302 with fungicidal and insecticidal activity was obtained. By active tracking and large-scale fermentation, the active compound was obtained and identified as Viridicatumtoxin A by LC-MS and X-Ray diffraction. Viridicatumtoxin A was found to show fungicidal activities to several agricultural pathogenic bacteria, and insecticidal activity to Plutella xylostella by further bioassay.

关 键 词：真菌 Viridicatumtoxin A 抗菌活性 杀虫活性 

分 类 号：R284[医药卫生—中药学]