果园螟蛾总科部分种类DNA条形码鉴定  

DNA barcoding of some pyraloid moths(Lepidoptera: Pyraloidea)in an apple orchard

作　　者：武宇鹏[1,2] 武春生 张萌[3] 苏田娟 朱朝东[2] Wu Yupeng;Wu Chunsheng;Zhang Meng;Su Tianjuan;Zhu Chaodong(Taiyuan University of Science and Technology,Taiyuan 030024,Shanxi Province,China;Key Laboratory of Zoological Systematics and Evolution,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China;Shanxi Plant Protection and Plant Quarantine Station,Taiyuan 030001,Shanxi Province,China)

机构地区：[1]太原科技大学,太原030024 [2]中国科学院动物研究所动物进化与系统学院重点实验室,北京100101 [3]山西省植物保护植物检疫总站,太原030001

出　　处：《植物保护学报》2018年第4期705-715,共11页Journal of Plant Protection

基　　金：山西省自然科学基金(2015011077);太原科技大学博士科研启动基金(20142031)

摘　　要：为检验DNA条形码在鳞翅目螟蛾总科蛾类鉴定中的可行性,对采自山西省太原市晋源区螟蛾总科26种78头蛾类标本分别提取了DNA,扩增了全部78头标本的线粒体cox1基因和其中75头标本的核糖体28S基因,并通过构建系统发育树、计算遗传距离及种间差异阈值等方法,对所有标本进行了鉴定和比较分析,检验了国际DNA条形码数据库BOLD(the barcode of life data)系统的鉴定成功率。结果表明,基于cox1基因和28S基因的系统发育树鉴定成功率分别为100.00%和97.14%,BOLD系统的鉴定成功率达到了67.94%。基于最大简约法、邻接法和最大似然法构建的系统发育树,鉴定结果均相同。基于cox1基因的种内遗传距离全部小于1.00%,种内种间的遗传距离形成明显的3.00%阈值现象。研究表明,cox1及28S基因均适用于供试螟蛾总科种类的鉴定,核糖体28S基因可以作为DNA条形码鉴定的辅助基因;BOLD系统数据库仍有待充实,且标本鉴定工作相对滞后;不同聚类分析方法对结果影响很小,其中邻接法计算速度快,更适合DNA条形码大数据的分析。In order to investigate the accuracy of DNA barcoding in identifying pyraloid moths of Lepidoptera, 78 samples（belonging to 26 species of pyraloids） from an apple orchard in Jinyuan District of Taiyuan were collected. All samples were used for DNA extraction. The genes of cox1 and 28 S were successfully amplified for all of the 78 samples, except for the 28 S genes of three samples. The sequences were used to construct phylogenetic trees, analyze the genetic distance and threshold, and test the identification success rate of the barcode of life data（BOLD） system. The results showed that the success rate according to the phylogenetic analysis using the genes of cox1 and 28 S were 100.00% and97.14%, respectively. The success rate of BOLD system was 67.94%. Furthermore, the results from the phylogenetic trees based on different methods（maximum parsimony, neighbor-joining and maximum likelihood） were consistent. The intra-species genetic distance was less than 1.00% in all species. There was an obvious 3.00% threshold between the intra-species and the inter-species genetic distances. The results indicated that cox1 and 28 S genes were suitable for DNA identification of the tested pyraloid moths, and 28 S gene could be used as an auxiliary gene for DNA barcoding; the BOLD system database still need to be enriched, and the specimen identification was somewhat lagging behind; different clustering methods only had a little influence on the result, and the neighbor-joining method was more suitable for the analysis of DNA barcoding for its faster calculation.

关 键 词：果园 螟蛾总科 DNA条形码 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]