菌株NDBJJ-BFG的鉴定及其对马铃薯甲虫不同虫态的毒力测定  

Identification and virulence of a fungal entomopathogen strain NDBJJ-BFG to Colorado potato beetle Leptinotarsa decemlineata(Say)

作　　者：段玉林 吴辉[2] 马志燕 杨柳 段晓东[4] 马德英 Duan Yulin;Wu Hui;Ma Zhiyan;Yang Liu;Duan Xiaodong;Ma Deying(Key Laboratory of the Pest Monitoring and Safety Control of Crops and Forests of the Xinjiang,College of Agronomy,Xinjiang Agricultural University,Urumqi 830052,Xinjiang Uygur Autonomous Region,China;Medical College Animals,Xinjiang Agricultural University,Urumqi 830052,Xinjiang Uygur Autonomous Region,China;Agricultural Technology Extension Center of Changshanzi Town,Urumqi 831402,Xinjiang Uygur Autonomous Region,China;Agricultural Technology Extension Center of Urumqi City,Urumqi 830000,Xinjiang Uygur Autonomous Region,China)

机构地区：[1]新疆农业大学农学院农林有害生物监测与安全防控重点实验室,乌鲁木齐830052 [2]新疆农业大学动物医学学院,乌鲁木齐830052 [3]乌鲁木齐市米东区长山子镇农业技术推广站,乌鲁木齐831402 [4]乌鲁木齐市农业技术推广中心,乌鲁木齐830000

出　　处：《植物保护学报》2018年第4期751-758,共8页Journal of Plant Protection

基　　金：国家公益性行业(农业)科研专项(201102034-02)

摘　　要：为筛选出对马铃薯甲虫Leptinotarsa decemlineata(Say)具有防治潜力的菌株,本研究从马铃薯甲虫僵虫虫体上分离菌株NDBJJ-BFG,通过形态学特征和分子生物学方法明确其分类地位,采用喷雾法和覆土法测定了不同浓度下该菌株对马铃薯甲虫室内毒力的致死中浓度LC50及半致死时间LT50。结果表明,经形态特征与r DNA ITS序列分析最终确定菌株NDBJJ-BFG为球孢白僵菌Beauveria bassiana(Bals.)Vuill。该菌株对1、2、3和4龄马铃薯甲虫幼虫的LC50分别为0.91×10~6、1.51×10~6、5.09×10~6和6.84×10~6个/m L;采用喷雾法和覆土法处理蛹的LC50分别为1.43×10~7个/m L和8.15×10~6个/m L,成虫的LC50分别为5.08×10~7个/m L和2.97×10~7个/m L。在孢子悬浮液浓度相同时LT50随虫龄的增大而延长,其中成虫的LT50最长,其次是蛹;在相同龄期下LT50随着孢子悬浮液浓度的增大而缩短。表明菌株NDBJJ-BFG对马铃薯甲虫1龄和2龄幼虫具有高毒力,其在马铃薯甲虫的生物防治中将具有较大的应用潜力。To screen the control potential strain to control Colorado potato beetle, Leptinotarsa decemlineata（Say）, the strain NDBJJ-BFG was isolated from L. decemlineata infected by disease. The classification of the strain NDBJJ-BFG was determined with both morphological and molecular biological methods, and the LC50 and LT50 of L. decemlineata larvae were monitored using spraying and covering with soil. The strain NDBJJ-BFG was identified as Beauveria bassiana（Bals.） Vuill based on morphological characteristics and r DNA ITS sequence analysis. LC50 values of the 1 st instar, 2 nd instar, 3 rd instar and 4 th instar larvae were 9.67 × 105, 1.51 × 10^6, 5.09 × 10^6 and 6.84 × 10^6 spores/m L, respectively. LC50 values of the pupae of spraying treatment and soil treatment were 1.43×10^7 spores/m L and 8.15×10^6 spores/m L,respectively, and LC50 values of adult were 5.08×10^7 spores/m L and 2.97×108 spores/m L, respectively.LT50 value increased along with larva instar increasing at the same concentration of spore suspension,and LT50 value of the adult was the highest, then followed by pupae. At the same larva instar, LT50 value decreased along with the concentration of spore suspension increasing. Therefore, the strain NDBJJBFG exhibited high virulence to both the 1 st and 2 nd instar larvae of L. decemlineata, and had high potential ability for the biological control of L. decemlineata.

关 键 词：马铃薯甲虫 球孢白僵菌 分离鉴定 毒力测定 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]