3D打印制备聚己内酯/Ⅰ型胶原组织工程半月板支架及其理化特性的研究  

Study on the preparation of polycaprolactone/type Ⅰ collagen tissue engineered meniscus scaffold by three-dimensional printing and its physiochemical properties

作　　者：沈师 陈明学[2] 高爽[2] 郭维民[2] 王振勇 李浩江 李旭[2] 张彬 鲜海[1,2] 张学亮 刘舒云[2] 郝立波[2] 卓乃强[1] 郭全义 SHEN Shi;CHEN Mingxue;GAO Shuang;GUO Weimin;WANG Zhenyong;LI Haojiang;LI Xu;HANG Bin;XIAN Hai;ZHANG Xueliang;LlU Shuyun;HAO Libo;ZHUO Naiqiang;GUO Quanyi(Department of Orthopedics and Joint Surgery,the Affiliated Hospital of Southwest Medical University,Luzhou Sichuan,646000 P.R.China;Institute of Orthopedics,General Hospital of Chinese PLA,Beijing Key Laboratory of Regenerative Medicine in Orthopedics,Key Laboratory of Musculoskeletal Trauma ＆ War Injuries,PLA,Beijing,100853,P.R.China)

机构地区：[1]西南医科大学附属医院骨与关节外科,四川泸州646000 [2]中国人民解放军总医院骨科研究所骨科再生医学北京市重点实验室全军骨科战创伤重点实验室,北京100853

出　　处：《中国修复重建外科杂志》2018年第9期1205-1210,共6页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家重点研发计划(2017YFC1103404); 国家自然科学基金面上项目(81772319);国家自然科学基金重点项目(21134004); 北京市自然科学基金面上项目(7172203)

摘　　要：目的采用低温沉积技术3D打印制备聚己内酯(polycaprolactone,PCL)/Ⅰ型胶原组织工程半月板支架(以下简称PCL/Ⅰ型胶原半月板支架),探讨其理化特性。方法制备15%PCL/4%Ⅰ型胶原溶液及15%PCL溶液,利用低温沉积技术3D打印制备PCL/Ⅰ型胶原半月板支架及PCL半月板支架。大体及扫描电镜观察支架形态及微观结构,生物力学试验测量支架压缩模量及拉伸模量,红外光谱分析支架成分,测量支架表面接触角;将两种支架及其浸提液分别与兔半月板细胞复合培养,细胞计数试剂盒8(cell counting kit 8,CCK-8)检测细胞增殖,并以正常培养细胞作对照;扫描电镜观察支架-细胞复合物中细胞黏附及生长情况。结果大体及扫描电镜观察显示,两种支架均具有取向的三维微观结构及孔隙,但PCL/Ⅰ型胶原半月板支架表面更粗糙。生物力学测试,两种支架压缩模量及拉伸模量比较,差异均无统计学意义(P>0.05)。红外光谱分析提示,PCL/Ⅰ型胶原半月板支架中PCL和Ⅰ型胶原成功混合。PCL/Ⅰ型胶原半月板支架表面接触角为(83.19±7.49)°,较PCL半月板支架(111.13±5.70)°显著减小(t=6.638,P=0.000)。CCK-8检测显示,随培养时间延长,两种支架浸提液培养的细胞数量呈递增趋势,与对照组比较差异均无统计学意义(P>0.05)。支架-细胞复合物扫描电镜观察示,PCL/Ⅰ型胶原半月板支架表面黏附细胞多于PCL半月板支架。结论低温沉积技术3D打印制备的PCL/Ⅰ型胶原半月板支架具有优良的理化学性能,无细胞毒性,有望作为半月板组织工程支架材料。Objective To manufacture a polycaprolactone（PCL）/type Ⅰ collagen（COL Ⅰ） tissue engineered meniscus scaffold（hereinafter referred to as PCL/COL Ⅰ meniscus scaffold） by three-dimensional（3 D） printing with low temperature deposition technique and to study its physicochemical properties. Methods First, the 15% PCL/4% COLⅠcomposite solution and 15% PCL simple solution were prepared. Then, 15% PCL/4% COL Ⅰmeniscus scaffold and 15%PCL meniscal scaffold were prepared by using 3 D printing with low temperature deposition techniques. The morphology and microstructure of the scaffolds were observed by gross observation and scanning electron microscope. The compression modulus and tensile modulus of the scaffolds were measured by biomechanical test. The components of the scaffolds were analyzed by Fourier transform infrared spectroscopy（FTIR）. The contact angle of the scaffold surfacewas measured. The meniscus cells of rabbits were cultured with the two scaffold extracts and scaffolds, respectively.After cultured, the cell proliferations were detected by cell counting kit 8（CCK-8）, and the normal cultured cells were used as controls. Cell adhesion and growth of scaffold-cell complex were observed by scanning electron microscope.Results According to the gross and scanning electron microscope observations, two scaffolds had orientated 3 D microstructures and pores, but the surface of the PCL/COLⅠ meniscus scaffold was rougher than the PCL meniscus scaffold. Biomechanical analysis showed that the tensile modulus and compression modulus of the PCL/COL Ⅰ meniscus scaffold were not significantly different from those of the PCL meniscus scaffold（P〉0.05）. FTIR analysis results showed that COL Ⅰ and PCL were successful mixed in PCL/COL Ⅰ meniscus scaffolds. The contact angle of PCL/COLⅠmeniscus scaffold [（83.19±7.49）°] was significantly lower than that of PCL meniscus scaffold [（111.13±5.70）°]（t=6.638,P=0.000）. The results of the CCK-8 assay indicated that with time,

关 键 词：半月板组织工程 低温沉积技术 3D打印技术 支架材料 理化特性 

分 类 号：R318.08[医药卫生—生物医学工程] TP391.73[医药卫生—基础医学]