金属络合法纯化苦碟子黄酮工艺  

Purification of Flavonoids from Ixeris Sonchifolia by Metal Complex Method

作　　者：郭真真 张文颖[1] 邹敬韬 王东[1] 王芳[3] 于青 代英辉[1] 刘东春[1] GUO Zhen-zhen;ZHANG Wen-ying;ZOU Jing-tao;WANG Dong;WANG Fang;YU Qing;DAI Ying-hui;LIU Dong-chun(School of Traditional Chinese Medicine,Shenyang Pharmaceutical University,Shenyang 110016,China;Tonghua Huaxia Pharmaceutical Co.,Ltd.,Tonghua 134100,China;School of Life Science and Biopharmaceutics,Shenyang Pharmaceutical University,Shenyang 110016,China)

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016 [2]通化华夏药业有限责任公司,吉林通化134100 [3]沈阳药科大学生命科学与生物制药学院,辽宁沈阳110016

出　　处：《现代中药研究与实践》2018年第4期49-51,共3页Research and Practice on Chinese Medicines

基　　金：吉林省科技发展计划资助项目(20130727010YY)

摘　　要：目的采用金属络合的方法分离纯化苦碟子中的黄酮类活性成分。方法以Mg^(2+)、Ca^(2+)、Zn^(2+)作为络合剂,以木犀草素-7-O-β-D-葡萄糖醛酸苷(Luteolin-7-O-β-D-glucuronide,LGU)为指标成分,HPLC-UV法测定LGU的含量和转移率,考察不同pH(8.0、9.0、9.5、10.0、11.0)条件下各金属离子对苦碟子黄酮的纯化效果。结果 pH=9.5时,Mg^(2+)络合物中LGU含量最高,与纯化前的醇提取物相比,含量提高5倍达到12.1%。结论采用金属络合法纯化苦碟子黄酮是一种简单、有效的纯化方法。Objective To study the purification technology of flavonoid from the Ixeris sonchifolia（EIS） by metal complex method. Methods Luteolin-7-O-β-D-glucuronide（LGU） as the key parameters, the contents and transfer rates in different metal complex（Mg2＋, Ca2＋ or Zn2＋） prepared at various p H of 8.0, 9.0, 9.5, 10.0 and 11.0 are investigated by HPLC-UV method. Results The results indicated that the complexation by Mg2＋ at pH 9.5 is the optimum. With this optimum condition, the content of LGU in Mg2＋ complex is 12.1 %, about 5 times as much that in EIS. Conclusion Purification of flavonoid Ixeris sonchifolia by metal chelating method is simple and effective.

关 键 词：抱茎苦荬菜 黄酮 金属络合 木犀草素-7-O-β-D-葡萄糖醛酸苷 分离纯化 

分 类 号：R283[医药卫生—中药学]