HPLC法测定益坤宁酊中梓醇、麦角甾苷、马替诺皂苷、氧化芍药苷、芍药内酯苷、芍药苷和α-香附酮  

Determination of catalpol, acteoside, martinoside, oxypaeoniflorin, albiflorin, paeoniflorin and α-cyperone in Yikunning Tincture by HPLC

作　　者：辛振杰 李庆国[2] XIN Zhen-jie;LI Qing-guo(Department of Pharmacy,Luoding Hospital of Traditional Chinese Medicine,Luoding 527200,China;School of Pharmaceutical Sciences,Guangzhou University of Chinese Medicine,Guangzhou 510006,China)

机构地区：[1]罗定市中医院药学部,广东罗定527200 [2]广州中医药大学中药学院,广东广州510006

出　　处：《现代药物与临床》2018年第8期1875-1878,共4页Drugs & Clinic

基　　金：广东省中医药强省项目(20142167)

摘　　要：目的建立HPLC波长切换法测定益坤宁酊中梓醇、麦角甾苷、马替诺皂苷、氧化芍药苷、芍药内酯苷、芍药苷和α-香附酮的方法。方法采用Agilent Eclipse XDB-C18色谱柱(250 mm×4.6 mm,5μm);流动相A:乙腈–甲醇(1∶3),流动相B:0.2%磷酸溶液,梯度洗脱;检测波长210 nm(梓醇)、330 nm(麦角甾苷、马替诺皂苷)和230 nm(氧化芍药苷、芍药内酯苷、芍药苷、α-香附酮);体积流量0.8 m L/min;柱温25℃;进样量10μL。结果梓醇、麦角甾苷、马替诺皂苷、氧化芍药苷、芍药内酯苷、芍药苷和α-香附酮在2.66~66.50、1.59~39.75、0.76~19.00、1.38~34.50、7.02~175.50、9.91~247.75、1.19~29.75μg/m L线性关系良好。平均加样回收率分别为97.97%、98.31%、96.99%、97.78%、99.28%、100.03%、97.43%,RSD值分别为1.42%、0.92%、1.30%、1.13%、1.08%、0.65%、1.27%。结论本法操作便捷、数据准确、灵敏度高,重复性好,可为益坤宁酊的质量控制提供参考。Objective To develop an HPLC with wavelength switching method for determination of catalpol, acteoside, martinoside, oxypaeoniflorin, albiflorin, paeoniflorin and α-cyperone in Yikunning Tincture. Methods The separation was performed on Agilent Eclipse XDB-C18 column（250 mm × 4.6 mm, 5 μm）. The mobile phase consisted of mobile phase A acetonitrile-methanol（1∶3） and mobile phase B 0.2% phosphoric acid solution with gradient elution. The detection wavelengths were set at 210 nm（catalpol）, 330 nm（acteoside and martinoside）, and 230 nm（oxypaeoniflorin, albiflorin, paeoniflorin, and α-cyperone）. The flow rate was 0.8 m L/min, temperature of column was set at 25 ℃, and volume of injection was 10 μL. Results The linear ranges of catalpol, acteoside, martinoside, oxypaeoniflorin, albiflorin, paeoniflorin, and α-cyperone were 2.66 - 66.50, 1.59 - 39.75, 0.76 - 19.00, 1.38 - 34.50, 7.02 - 175.50, 9.91 - 247.75, and 1.19 -29.75 μg/m L, respectively. The average recoveries were 97.97%, 98.31%, 96.99%, 97.78%, 99.28%, 100.03%, and 97.43% with RSD 1.42%, 0.92%, 1.30%, 1.13%, 1.08%, 0.65%, and 1.27%, respectively. Conclusion The method is convenient, accurate, sensitive, and reproducible, which can provide reference for quality control of Yikunning Tincture.

关 键 词：益坤宁酊 梓醇 麦角甾苷 马替诺皂苷 氧化芍药苷 芍药内酯苷 芍药苷 Α-香附酮 高效液相色谱 

分 类 号：R286.02[医药卫生—中药学]