HPLC法测定畅脉乐胶囊中芍药苷的含量  

Content Determination of Paeoniflorin in ChangMaiLe Capsule by HPLC

作　　者：翁一玲[1] 陈松旺 李煌[3] WENG Yi-ling;CHEN Song-wang;LI Huang(The second Affiliated People＇s Hospital of Fujian Univer-sity of Traditional Chinese Medicine,Fuzhou 350003,China;Fujian medical equipment and pharmaceuti-cal packaging materials inspection institute,Fuzhou 350001,China;College of Pharmacy,Fujian Universi-ty of Traditional Chinese Medicine,Fuzhou 350122,China)

机构地区：[1]福建中医药大学附属第二人民医院药学部,福州350003 [2]福建省医疗器械与药品包装材料检验所,福州350001 [3]福建中医药大学药学院,福州350122

出　　处：《海峡药学》2018年第8期59-61,共3页Strait Pharmaceutical Journal

摘　　要：目的研究并建立高效液相色谱法测定畅脉乐胶囊中芍药苷的含量。方法本实验以乙腈︰0.1%磷酸水溶液=18∶82(v/v)为流动相;所用色谱柱为Diamonsi L C_(18)(2)(5μm,250×4.6mm);柱温:30℃,进样量:10μL,流速:1.0mL·min^(-1),检测波长为230nm。结果按以上实验方法,芍药苷在12.11μg·mL^(-1)~151.4μg·mL^(-1)(r=0.9997)浓度范围内与其峰面积具有良好的线性关系。芍药苷的平均回收率为97.40%,RSD值为1.69%(n=6)。结论所述方法结果准确,可以作为检测畅脉乐胶囊中芍药苷含量的方法并用于该制剂的质量控制。OBJECTIVE To research and establish a method for the determination of paeoniflorin in ChangMai Le capsule by HPLC. METHODS The mobile phase was consisted of the acetonitrile-0. 1% phosphoric acid solution（ 18∶ 82）. The chromatographic column was Diamonsil C_（18）（ 2）（ 5μm,250 × 4. 6 mm）. The column temperature was 30℃. The injection volume was 10μL. Detection was performed at 230 nm with eluent flow rate of 1 mL·min^（-1）. RESULTS According to the above experimental methods,the paeoniflorin have good linear relationship with the peak area in the range of 12. 11μg·mL^（-1）-151. 4μg·mL^（-1）（ r = 0. 9997）. While the average recovery of paeoniflorin was 97. 40%,and the RSD was 1. 69%（ n = 6）. CONCLUSION The method has an accurate result and can be used as a method for detecting the content of paeoniflorin in ChangMaiLe capsule. And used to control the quality of ChangMaiLe capsule.

关 键 词：畅脉乐胶囊 芍药苷 高效液相色谱法 含量测定 

分 类 号：R927[医药卫生—药学]