基于荷移反应的液-液微萃取-光度法测定电子烟烟液中烟碱含量  

Determination of Nicotine in E-Cigarettes Liquids by Liquid-Liquid Microextraction-Spectrophotometry Method Based on Charge Transfer Reaction

作　　者：吴亿勤 张承明 刘巍 李利君 杨柳 王昆淼 王蒙[2] 杨亚玲[2] WU Yiqin;ZHANG Chengming;LIU Wei;LI Lijun;YANG Liu;WANG Kunmiao;WANG Meng;YANG Yaling(Technology center of Yunnan tobacco industry limited liability company,Kunming 650231,China;Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,China)

机构地区：[1]云南中烟工业有限责任公司技术中心,云南昆明650231 [2]昆明理工大学生命科学与技术学院,云南昆明650500

出　　处：《昆明理工大学学报（自然科学版）》2018年第4期78-82,共5页Journal of Kunming University of Science and Technology(Natural Science)

基　　金：云南烟草化学重点实验室开放基金项目(2016539200340111);云南中烟工业公司重点项目(2017JC04)

摘　　要：建立了体液液微萃取(LLME)–分光光度法测定烟碱含量的方法.在酸性条件下,烟碱与氯醌酸络合发生荷移反应并生成有色络合物,以聚乙烯醇作为光增敏剂,疏水性离子液体1-辛基-3-甲基咪唑六氟磷酸盐([C8MIM][PF6])作为萃取剂,涡旋混合后结合分光光度法对烟碱进行测定.研究了酸度、离子强度、萃取剂种类、萃取剂用量、萃取时间等条件对测定的影响.实验结果表明:在最优条件下,方法的线性范围为0.5~80.0μg/m L(R2=0.999 6),检出限为0.3μg/m L,加标回收率为92.5%~103.0%,相对标准偏差为1.4%~2.9%.本方法简单快速,灵敏度高,可广泛应用于电子烟烟液中烟碱的测定.Liquid-liquid micro-extraction（LLME）-spectrophotometry method was developed for the determination of nicotine in e-cigarettes liquids. In acidic conditions,nicotine reacts with chloroquinonic acid to undergo charge transfer reaction and produce colored complexes. Polyvinyl alcohol was used as light sensitizer,and hydrophobic ionic liquid 1-octyl-3-methylimidazolium hexafluoride phosphate（[C8 MIM][PF6]） was used as extraction solvent,then the mixed organic phase containing ionic liquid and colored complexes was determined by spectrophotometry. Extraction conditions such as the sample pH,ionic strength,categories and amount of extraction solvents and extraction time were studied and optimized. The experimental results showed that under the optimal experimental conditions,a good linearity for the analysis of nicotine in the concentration range of 0. 5 ~ 80. 0μg/m L（R^2= 0. 999 6） was obtained,the limit of detection（LOD） was 0. 3 μg/m L,and the recoveries were92. 5% ~ 103. 0% with the relative standard deviations of 1. 4% ~ 2. 9%. The proposed method is simple,rapid and sensitive,and can be widely applied to the determination of nicotine in e-cigarettes liquids.

关 键 词：烟碱 荷移反应 离子液体 液液微萃取 分光光度法 

分 类 号：TS458[农业科学—烟草工业] R917[医药卫生—药物分析学]