水杨酸调控敏感型黑大豆抗铝性研究  

Regulating Mechanism of Salicylic Acid on Aluminum Resistance of Sensitive Black Soybeans

作　　者：李芳 滕建晒 陈健[2] 彭亮 于丽娟[4] 陈宣钦[1] LI Fang;TENG Jianshai;CHEN Jian;PENG Liang;YU Lijuan;CHEN Xuanqin(Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650504,China;Institute of Food Quality and Nutrition,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;Forestry Bureau of Xinyu,Xinyu 338000,China;Institute of Food Crops,Yunnan Academy of Agricultural Sciences,Kunming 650231,China)

机构地区：[1]昆明理工大学生命科学与技术学院,云南昆明650504 [2]江苏省农业科学院农产品质量安全与营养研究所,江苏南京210014 [3]江西省新余市林业局,江西新余338000 [4]云南省农业科学院粮食作物研究所,云南昆明650231

出　　处：《昆明理工大学学报（自然科学版）》2018年第4期83-92,共10页Journal of Kunming University of Science and Technology(Natural Science)

基　　金：国家自然科学基金地区科学基金项目(31460058;31360058);江苏省农业科技自主创新资金项目[CX(14)2096]

摘　　要：抗性作物品种中水杨酸(salicylic acid,SA)信号与铝(aluminum,Al)抗性相关,但对于SA调控敏感作物抗Al胁迫的能力还有待评估和研究.本实验选用云南本地Al敏感型黑大豆根系为材料,研究了SA对Al胁迫下根内生理生化的调控作用,结果显示:(1)Al Cl3处理抑制根生长并呈现浓度效应,30μM Al Cl3处理可导致中等程度的抑制效应;(2)100μM SA处理可最大程度地缓解Al Cl3(30μM)对根长的抑制效应;(3)SA显著抑制Al胁迫下根尖内Al的累积、丙二醛含量、细胞死亡、活性氧累积(包括过氧化氢和超氧阴离子);(4)Al胁迫下根尖内一系列抗氧化酶基因(Cu-Zn SOD1、Cu-Zn SOD2、Cu-Zn SOD3、CAT3、CAT4、CAT5、PRX2B、PRX4)的表达维持不变或轻微上调,而加入SA后则大幅度上调了这些基因的表达水平;(5)聚类分析结果显示,SA能够逆转Al胁迫对伤害性生理指标的刺激效应;SA调控下Al损伤途径与Al抗性途径呈现负相关性.上述揭示出SA具备显著提升Al敏感型黑大豆抵抗Al胁迫的能力,这与其高效激活抗氧化系统密切相关.这为Al污染地区常规栽培品种的安全生产调控提供了一定的科学依据.Salicylic acid（SA） signal has been related to aluminum（Al） tolerance in Al-resistant cultivar,but the capability of SA-regulated Al tolerance in Al-sensitive cultivar needs to be further evaluated. In this study,roots of Al-sensitive black beans（a local cultivar in Yunnan Province） were selected to investigate the effect of SA on the intrinsic physiological regulation under Al stress. Basically,five lines of results were obtained as follows.（1） Al Cl3 treatment significantly inhibited root growth in a dose-dependent manner. Al Cl3 at 30 μM induced moderate inhibition of root elongation.（2） Treatment with SA at 100 μM showed most alleviated effect on root inhibition induced by Al Cl3（30 μM）.（3） SA treatment remarkably decreased Al accumulation,MDA content,cell death,ROS accumulation（including hydrogen peroxide and superoxide radical）in root tip under Al stress.（4） Compared to control,the expression level of a serious of anti-oxidative genes（Cu-ZnSOD1,Cu-ZnSOD2,Cu-ZnSOD3,CAT3,CAT4,CAT5,PRX2 B,PRX4） showedunchanged or slightly increased in Al-treated root tip. However,the addition of SA pronouncedly induced the increase in the expression level of these genes.（5） Hierarchical cluster analysis suggested that SA reversed the inducible effect of Al on root injury indexes. Al toxicity was negatively related to Al-resistant pathway in root tip under the regulation of SA. All the above results revealed that SA had the ability to confer Al tolerance in Al-sensitive black bean,which was closely linked to the capability of SA in the stimulation of anti-oxidative capacity.This study would provide a scientific basis for the regulation of safety production of conventional cultivar in Al-contaminated area.

关 键 词：水杨酸 铝胁迫 敏感型黑大豆 抗氧化物酶系统 

分 类 号：Q945.78[生物学—植物学]