西瑁岛软珊瑚Sinularia sp.的化学成分和生物活性研究  

The study on the chemical constituents and bioactivities of soft coral Sinularia sp. from Ximao Island

作　　者：李松威 姚励功 梁林富[3] 郭跃伟 LI Song-wei;YAO Li-gong;LIANG Lin-fu;GUO Yue-wei(School of Pharmacy,Nanchang University,Nanchang 330006,China;State Key Laboratory of Drug Research,Shanghai Institute of MateriaMedica,Chinese Academy of Sciences,Shanghai 201203,China;College of Materials Science and Engineering,Central South University of Forestry and Technology,Changsha 410004,China;Open Studio for Drugability Research of Marine Natural Products,Qingdao National Laboratory for Marine Science and Technology,Qingdao 266237,China)

机构地区：[1]南昌大学药学院,江西南昌330006 [2]中国科学院上海药物研究所新药研究国家重点实验室,上海201203 [3]中南林业科技大学材料科学与工程学院,湖南长沙410004 [4]青岛海洋科学与技术国家实验室海洋先导物成药性研究开放工作室,山东青岛266237

出　　处：《中国海洋药物》2018年第4期1-6,共6页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金项目(81520108028;21672230;41506187);国家自然科学基金--山东联合基金项目(U1606403);上海市科委基金项目(15431901000);新药研究国家重点实验室项目(SIMM 1705ZZ-01)资助

摘　　要：目的对采自中国南海西瑁岛的软珊瑚Sinulariasp.的化学成分和生物活性进行研究。方法综合利用薄层色谱、硅胶柱色谱、凝胶柱层析、半制备HPLC等分离手段,结合NMR和MS等波谱数据分析并对比文献数据,对化合物进行分离鉴定;选用A549和HL-60细胞株,采用CCK-8筛选方法,对化合物进行体外细胞毒活性测试。结果从软珊瑚Sinulariasp.中共分离鉴定了10个甾体化合物,包括8个孕甾烷及其苷类化合物:3β-羟基-5,20-二烯孕甾烷乙酸酯(1),20-烯-3-酮孕甾烷(2),1,20-二烯-3-酮孕甾烷(3),1,4,20-三烯-3-酮孕甾烷(4),3β-羟基-20-烯孕甾烷(5),3β-羟基-5,20-二烯孕甾烷(6),1,4-二烯-3-酮-20α-羟基孕甾烷乙酸酯(7),3β-羟基-5,20-二烯孕甾烷吡喃岩藻糖苷(8);1个麦角甾烷类化合物:(22E,24R)-3β,5α,6β-三羟基-7,22-二烯麦角甾烷(9)和1个胆甾烷类化合物:(20S)-1,4-二烯-3,16-二酮-20β-羟基胆甾烷(10)。结论化合物1~10对A549细胞株均未表现明显的细胞毒活性,但化合物4和7对HL-60细胞株具有中等的细胞毒活性,其IC_(50)值分别为1.61和3.26μmol/L。Objective To study the chemical constituents and bioactivities of soft coral sinulariasp.collected from Ximao Island in South China Sea.Methods The isolation and purification of these compounds were performed by TLC,silica gel,Sephadex LH-20 column chromatography and semi-preparative HPLC.Their structures were characterized by analysis of NMR and MS data and comparison with the data reported in literatures.The cytotoxicity of these compoundsagainst A549 and HL-60 cell lines hasbeen tested by using Cell Counting Kit-8（CCK-8）method.Results Ten known steroids were isolated from the soft coral sinulariasp.and their structures were identified as pregna-5,20-dien-3β-O-acetate（1）,pregna-20-en-3-one（2）,pregna-1,20-dien-3-one（3）,pregna-1,4,20-trien-3-one（4）,pregna-20-en-3β-ol（5）,pregna-5,20-dien-3β-ol（6）,pregna-1,4-dien-3-one-20α-O-acetate（7）,α-galactopyranoside-3β-pregna-5,20-dien-3-yl-6-deoxy（8）,（22 E,24 R）-ergosta-7,22-dien-3β,5α,6β-triol（9）and（20 S）-20-hydroxy cholost-1,4-dien-3,16-dione（10）.Conclusion Though compounds 110 didn’texhibit potent cytotoxicity against A549 cell lines,compounds 4 and 7 showed moderate cytotoxicity against HL-60 cell lines with IC50 values of 1.61 and 3.26μmol/L,respectively.

关 键 词：软珊瑚 甾体 细胞毒 

分 类 号：R931[医药卫生—生药学]