海洋来源真菌Penicillium sp. wqh-572的次级代谢产物研究  

Isolation and identification of the secondary metabolites from marine-derived fungus Penicillium sp. wqh-572

作　　者：李鑫 吴祺豪 王碧霞 陈苏[1] 陈建伟 章华伟[1] 王鸿[1] LI Xin;WU Qi-hao;WANG Bi-xia;CHEN Su;CHEN Jian-wei;ZHANG Hua-wei;WANG Hong(College of Pharmaceutical Science,Zhejiang University of Technology,Hangzhou 310014,China)

机构地区：[1]浙江工业大学药学院,浙江杭州310014

出　　处：《中国海洋药物》2018年第4期12-16,共5页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金面上项目(81773628;41776139);国家自然科学基金应急管理项目(81741165);浙江省自然科学基金项目(LY16H300008;LY16H300007)资助

摘　　要：目的研究浙江普陀岛海泥来源真菌Penicilliumsp.wqh-572的次级代谢产物及其抑菌活性。方法将菌株进行大规模发酵,发酵液以乙酸乙酯萃取获得粗提物,采用小孔树脂MCI柱层析色谱和半制备型高效液相色谱等分离纯化方法从粗提物中得到化合物,通过核磁(1 H NMR和13 C NMR)和质谱(MS)等波谱分析方法,并与相关文献比较,鉴定化合物结构。结果从该真菌发酵液的乙酸乙酯萃取相中分离得到6个化合物,鉴定结果分别为physcion(1)、emodin(2)、7-hydroxy-2-(2-hydroxypropyl)-5-methylchromone(3)、isorhodoptilometrin(4)、2-(2’,4’,6’-trihydroxyphenyc)-(7-hydroxy-5-methyc)chromone(5)及(Z)-N-(4-hydroxy styryl)formamide(6)。其中,其中化合物5为首次从青霉属真菌中分离得到。化合物1对3种植物病原菌均具有抑制作用。Objective To study the secondary metabolites of Penicilliumsp.wqh-572.Methods The ethyl acetate extract of the fermented liquid was purified after large scale fermentation,MCI chromatography and HPLC were used to isolate the compounds from the extract.The structures of the compounds were identified on the basis of nuclear magnetic resonance（1 H NMR and 13 C NMR）,mass spectrum（MS）and the related literature.Results Six compounds were isolated from the ethyl acetate extract of P.oxalicum wqh-572.The structures were identified as physcion（1）,emodin（2）,7-hydroxy-2-（2-hydroxypropyl）-5-methylchromone（3）,isorhodoptilometrin（4）,2-（2’,4’,6’-trihydroxyphenyc）-（7-hydroxy-5-methyc）chromone（5）and（Z）-N-（4-hydroxy styryl）formamide（6）,respectively.Compound1 exhibited inhibitory activity against three strains of plant pathogenic fungi.

关 键 词：海洋真菌 青霉属 次级代谢产物 

分 类 号：R931[医药卫生—生药学]