CD32抗体诱导产生的人CRP对小鼠急性肾损伤自噬的研究  

Effect of Human C- reactive Protein Induced by Anti-CD32 Antibody on Autophagy of Acute Kidney Injury in Mice

作　　者：嵇金陵 汤钱球 张小云[1] JI Jin-ling;TANG Qian-qiu;ZHANG Xiao-yun(Department of Clinical Laboratory,the Huaian First Hospital Affiliated to Nanjing Medical University,Huaian 223300,Jiangsu,China)

机构地区：[1]南京医科大学附属淮安第一医院检验科,江苏淮安223300

出　　处：《医学临床研究》2018年第8期1513-1516,共4页Journal of Clinical Research

摘　　要：[目的]探讨人C-反应蛋白（C-reactive protein,CRP）对急性肾损伤（AKI）自噬的影响.[方法]在术前2 h经腹腔注射25μg/mL体血容量（体血容量等于小鼠的克质量乘以0.08）的抗CD32抗体诱导小鼠体内人CRP的产生,然后构建急性肾缺血-再灌注损伤小鼠模型,分Sham组、野生型小鼠组（CRP-Wt-I/R组）、表达人CRP基因的转基因小鼠组（CRP-Tg-I/R组）,比较肾缺血-再灌注损伤术前2h和术后0h、24h、72h小鼠体内CRP的变化,术后24h、72h时间点获取肾脏标本.HE染色后观察肾脏组织的病理变化,用免疫组化的方法检测自噬相关蛋白Beclin-1的表达情况,在透射电子显微镜下观察肾脏组织的自噬现象.[结果]小鼠肾缺血-再灌注损伤术后24 h、72 h时间点,CRP-Tg-I/R组肾小管上皮细胞Beclin-1蛋白的表达明显下降（P〈0.01）,看不到自噬小体.[结论]人CRP下调AKI肾脏组织的自噬活性.[Objective]To invest the effect of human C- reactive protein on autophagy in acute kidney inju- ry . [Methods] In order to induce the production of human CRP in mice, 25 9 g/mL body volume was injected intraperitoneally 2 hours before operation （body blood volume was equal to the gram weight of mice multiplied by 0.08 ） of anti CD32 antibody, then the acute renal ischemia-reperfusion injury model was established in mice. The mice were divided into Sham group, wild type mice group （CRP-Wt-I/R group） and transgenic mice expressing human CRP gene group（CRP-Tg-I/R group）; the changes of CRP in mice before and after renal ischemia-reperfusion injury 2 hours before and Oh,24h.72h after operation were compared, Kidney specimens were obtained at 24 h and 72 h after operation. After HE staining, the pathological changes of renal tissue were observed, the expression of autophagy associated protein Beclin-1 was detected by immunohistochemical meth- od, and the autophagy of renal tissue was observed under transmission electron microscope.[Results]At 24 hours and 72 hours after renal ischemia-reperfusion injury, the expression of Beclin-1 protein in renal tubular epithelial cells of the CRP-Tg-I/R group decreased significantly （ P〈0.01）, and no autophagy was observed. [Conclusion]Human CRP down-regulated autophagy activity in renal tissue of AKI.

关 键 词：肾疾病/治疗 疾病模型 动物 受体 IGG C反应蛋白质 

分 类 号：R965.1[医药卫生—药理学]