紫花苜蓿种质资源萌发期耐盐性鉴定  

Identification of Salt-tolerant Properties During Germination Period of Medicago sativa L. Germplasm Resources

作　　者：王静[1] 许兴[1] 麻冬梅[2] WANG Jing;XU Xing;MA Dongmei(School of Agriculture,Ningxia University,Yinchuan,the Ningxia Hui Autonomous Region 750021;Ecological Center,Ningxia University,Yinehuan,the Ningxia Hui Autonomous Region 750021)

机构地区：[1]宁夏大学农学院,宁夏回族自治区银川750021 [2]宁夏大学生态中心,宁夏回族自治区银川750021

出　　处：《核农学报》2018年第10期1939-1948,共10页Journal of Nuclear Agricultural Sciences

基　　金：牧草种质资源创新与新品种选育(NYYZ-2014-Z12)

摘　　要：为鉴定及评价紫花苜蓿种质资源萌发期耐盐性,以25份紫花苜蓿品种为试验材料,在种子萌发培养基中分别添加0(CK)、150、250和350 mmol·L-1NaCl进行持续胁迫处理,研究25个苜蓿品种的种子萌发期耐盐性相关指标。结果表明,苜蓿种子在第3、第7和第10天的相对发芽率和发芽势均随着NaCl胁迫浓度的增加呈降低趋势;盐害指数随着NaCl胁迫浓度的增加而升高;胚根长、胚芽长均随着NaCl胁迫浓度的增加而降低,且萌发时间相应延长;胚根、胚芽重均随着NaCl胁迫浓度的增加而逐渐减少,且生物量下降。25个苜蓿品种的种子萌发期耐盐性差异显著,其中苜蓿品种‘WL326GE’在350mmol·L-1NaCl胁迫处理下,发芽势为46.67%,相对发芽率为48.33%,生物量为最高,耐盐等级评价为高耐,萌发期耐盐性较强。本研究结果为适宜盐碱地种植的耐盐苜蓿品种的筛选提供了理论依据。The aim of present study was to identify and evaluate the salt tolerant traits of alfalfa germplasm resources at germination stage. In this paper,25 alfalfa cultivars were grown on the seed germination medium including 0（CK）,150,250 and 350 mmol· L^-1 NaCl for continuous stressed treatments,respectively. The salt-tolerance indexes were examined on 25 alfalfa cultivars at the germination stage. The result showed that the relative germination rate of alfalfa seed and germination potential during the period of the third day,seventh day and tenth day were decreased with increasing of NaCl concentration. The salt injury index was elevated with the increased NaCl concentration,whereas the radicle and embryo length were decreased with the extension of germination time. In addition,the weight of radical and embryo was reduced along with the increased NaCl concentration,which led to a decrease in biomass. There was significant extent of variation in salt-tolerant properties of alfalfa between different 25 varieties. Ⅰ was found that the germination energy of ‘WL326 GE＇alfalfa was 46. 67%,and the relative germination rate was 48. 33% with the highest biomass under the 350 mmol·L^-1 NaCl treatment. It was suggested that ‘WL326 GE ＇alfalfa was a highly resistant cultivar with a strong salt-tolerant properties. This study provides a theoretical basis for the selection of salt-tolerant alfalfa varieties suitable for saline-alkali cultivation.

关 键 词：紫花苜蓿 萌发期 耐盐性 鉴定及评价 

分 类 号：S541.9[农业科学—作物学]