不同树龄茶树根际土壤细菌多样性的T-RFLP分析  

Using T-RFLP technology to analyze bacterial diversity in the rhizospheric soils of tea tree at different ages

作　　者：王海斌[1,2] 陈晓婷[1] 丁力[1] 邱丰艳[1] 叶江华[3] 贾小丽[3] 孔祥海[1] WANG Haibin;CHEN Xiaoting;DING Li;QIU Fengyan;YE Jianghua;JIA Xiaoli;KONG Xianghai(College of Life Sciences,Longyan University,Longyan 364012,China;Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring,Fufian Agriculture and Forestry University,Fuzhou 350002,China;Wuyi University,Wayishan 354300,China)

机构地区：[1]龙岩学院生命科学学院,龙岩364012 [2]福建农林大学福建省农业生态过程与安全监控重点实验室,福州350002 [3]武夷学院,武夷山354300

出　　处：《应用与环境生物学报》2018年第4期775-782,共8页Chinese Journal of Applied and Environmental Biology

基　　金：国家重点研发计划项目(2016YFD0200900);国家948项目(2014-Z36);中国博士后科学基金(2016M600493);福建省自然科学基金青年基金(2017J05057);福建省中青年教师教育科研项目(JAT170573);龙岩学院人才引进项目(LB2015001)资助~~

摘　　要：以不同树龄铁观音茶树根际土壤为研究对象,采用末端限制性片段长度多样性（T-RFLP）技术分析茶树不同树龄对土壤细菌多样性的影响.结果显示,茶树根际土壤细菌Hae III和Msp I酶切产物的T-RFs片段数量及辛普森指数、香农威纳指数均随着茶树树龄增加呈现下降趋势.相关性分析结果表明,与茶树树龄呈显著或极显著正相关的细菌T-RFs片段9个,共34种细菌,由10个纲组成,按照功能可分成病原菌及与改善土壤质地、抑制病原菌、碳素循环、氮素循环、硫素循环等相关的微生物6大类,其中病原菌占比达58.82%.与茶树树龄呈显著或极显著负相关的细菌T-RFs片段17个,共38种细菌,由12个纲组成,按照功能可分成病原菌及与氮素循环、碳素循环、抑制病原菌、改善土壤质地等相关的细菌共5大类,其中除病原菌外,其余细菌占比达78.95%.综上所述,随着茶树树龄增加,茶树根际土壤细菌多样性及其功能发生了显著的变化,结果可为后期细菌群落互作效应研究提供一定理论基础.The rhizospheric soils of Tieguanyin at different ages were used as the study materials, and terminal-restriction fragment length polymorphism（T-RFLP） was used to analyze the changes in bacterial diversity. The results showed that the number of T-RFs, Simpson index, and Shannon index decreased significantly with the age of the tea tree. Results of correlation analysis showed that 9 T-RFs from the bacterial community were significantly and positively correlated with the age of the tea tree, and included 34 species of microbes belonging to 10 classes. The 34 microbes were divided into 6 types according to their functional attributes, and included pathogenic bacteria, bacteria that improved soil texture or inhibited pathogenic bacteria, and bacteria associated with the carbon, nitrogen, or sulfur cycles, in which the percentage of pathogenic bacteria was 58.82%. Seventeen T-RFs were significantly and negatively correlated with the age of the tea tree, and included 38 species of bacteria belonging to 12 classes. The 38 bacterial species were divided into 5 types according to their functional attributes, and included pathogenic bacteria, bacteria that improved soil quality or inhibited pathogenic bacteria, and bacteria associated with the carbon cycle or nitrogen cycle, in which all the others, barring the pathogenic bacteria, accounted for 78.95% of the bacterial population. In brief, the diversity and function of bacteria in the rhizospheric soil of tea tree changed significantly with the age of the tea tree, which provides a theoretical basis for studying the interactions of bacterial communities.

关 键 词：茶树 树龄 根际土壤 细菌多样性 T-RFLP 

分 类 号：S154.36[农业科学—土壤学] S571.1[农业科学—农业基础科学]