二氢杨梅素对辐射引起神经元凋亡的保护作用  

Protective Effects of Dihydromyricelin on Neuron Apoptosis Induced by Gamma-Radiation

作　　者：张捷[1] 张恩艳[1] 郭宏举 冯桂检 常李荣[1] ZHANG Jie;ZHANG En-yan;GUO Hong-ju;FENG Gui-jian;CHANG Li-rong(Department of Pharmacy,the 306th Hospital of PLA,Beijing 100101,China)

机构地区：[1]解放军306医院药学部,北京100101

出　　处：《解放军药学学报》2018年第4期292-295,300,共5页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金面上项目;No.30772631

摘　　要：目的探讨二氢杨梅素对γ射线辐射引起皮层神经元凋亡的保护作用和可能作用机制。方法原代培养的大鼠皮层神经元提前给予二氢杨梅素,1h后以8Gy辐射强度的γ射线照射10min。24h后MTT检测细胞存活率,试剂盒测试丙二醛和超氧阴离子的含量以及Caspase-9和Caspase-3的活性,Western blot检测细胞色素C、Bcl-2和Bax的表达。结果二氢杨梅素可剂量依赖性地提高γ射线辐射的细胞存活率,显著降低丙二醛和超氧阴离子的含量,抑制Caspase-9和Caspase-3的活性,抑制细胞色素C的释放以及Bcl2和Bax的比值,从而保护神经元。结论二氢杨梅素对γ射线引起的皮层神经元损伤有保护作用,这可能与其抑制氧化应激和细胞凋亡有密切关系。Objective To investigate the protective effects of dihydromyricelin（DHM）on neuron apoptosis induced by gamma-radiation.Methods Primarily cultured cortical neurons were obtained from rats which were 19 dpregnant.The neurons were pre-incubated with DHM for 1 hbefore being subjected to gamma-radiation for 10 min at an intensity of 8 Gy.The biomarkers related to oxidative stress and apoptosis,such as the contents of malondialdehyde（MDA）and superoxide anion,the activities of Caspase-9 and Caspase-3,and the expression of cytochrome c（Cyt C）as well as Bcl-2,Bax,were measured.Results DHM dose-dependently increased cell viabilities of neurons exposed to gamma-radiation.DHM significantly reduced the production of MDA and superoxide anion.DHM markedly inhibited the activities of Caspase-9 and Caspase-3.Furthermore,the expression of CytC in the mitochondria and the ratio of Bcl-2 to Bax were increased by DHM treatment.Conclusion DHM has potent neuroprotective effects against gamma-radiation,which maybe closely related to the anti-oxidative stress and apoptosis of DHM.

关 键 词：二氢杨梅素 辐射 氧化应激 细胞凋亡 

分 类 号：R965[医药卫生—药理学]