HPLC法同时测定消银散胶囊中胡薄荷酮和蛇床子素含量  

Determination of Pulegone and Osthole in Xiaoyinsan Capsules by HPLC

作　　者：何德云[1] 钟元高[2] 周涛[2] 杜建红[1] HE De-yun;ZHONG Yuan-gao;ZHOU Tao;DU Jian-hong(Institute for Drug and Instrument Control of Chengdu Military Region,Chengdu 610017,China;Bayi Orthopedic Hospital,Chengdu Military Region,Chengdu 610031,China)

机构地区：[1]成都军区联勤部药品仪器检验所,四川成都610017 [2]成都军区八一骨科医院,四川成都610031

出　　处：《解放军药学学报》2018年第4期348-350,共3页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的建立消银散胶囊中胡薄荷酮和蛇床子素的含量测定方法。方法色谱柱:岛津Wondasil C_(18)-WR(4.6mm×250mm,5μm),流动相:乙腈-水(60∶40),检测波长:300nm,流速:1.0 ml·min^(-1)。结果胡薄荷酮和蛇床子素分别在0.041 54~0.4154mg·ml^(-1)和0.003 552~0.035 52mg·ml^(-1)范围内与峰面积呈良好的线性关系,胡薄荷酮线性回归方程式为Y=979 954 X+15 164,r=0.9989(n=6);蛇床子素线性回归方程式为Y=3×107 X+552.13,r=1.000(n=6);胡薄荷酮平均回收率为100.29%,RSD为1.000%(n=6),蛇床子素平均回收率为100.01%,RSD为0.8%(n=6)。结论HPLC法可用于同时测定消银散中胡薄荷酮和蛇床子素的含量。Objective To establish a method for determination of pulegone and osthole in Xiaoyansan capsules using HPLC.Methods In the environment of HPLC,the column was Shimadzu Wondasil C18-WR（4.6 mm×250 mm,5μm）,while the mobile phase consisted of acetonitrile-water（60∶40）.The detection wavelength was 300 nm,and the flow rate was 1.0 ml·min-1.Results There was a good linear relationship with the peak area within the range of 0.041 54-0.4154 mg·ml-1 for pulegone and 0.003 552-0.035 52 mg·ml-1 for osthole.The linear regression equation was Y =979 954 X ＋15 164,r =0.9989（n =6）for pulegone,and Y =3×10^7 X ＋552.13,r =1.000（n =6）for osthole.The average recovery was 100.29%with an RSD of 1.0% for pulegone（n =6）,and 100.01% for osthole with an RSD of 0.8%（n =6）.Conclusion This HPLC method can be used for simultaneous determination of the contents of pulegone and osthole in Xiaoyansancapsules.

关 键 词：消银散胶囊 HPLC 胡薄荷酮 蛇床子素 含量测定 

分 类 号：R927.2[医药卫生—药学]