内蒙古敖汉旗地区乙肝患者HBV基因型分布研究  

HBV Genotypes Distribution among Patients Infected with HBV in Aohan Area of Inner Mongolia

作　　者：徐晓光 温小辉[2] 付婷[2] 高洁[2] 严敏 张维璐[2] XU Xiao-guan;WEN Xiao-hui;FU Ting;GAO Jie;YAN Min;ZHANG Wei-lu(Aohan hospital,Chifeng,Chifeng,Inner Mongolia,024300,China;Department of Epidemiology,School of Public Health,the Fourth Military Medical University,Xi＇an,Shaanxi,710032,China)

机构地区：[1]内蒙古赤峰市敖汉旗医院消化内科,内蒙古赤峰024300 [2]第四军医大学军事预防医学系流行病学教研室,陕西西安710032

出　　处：《现代生物医学进展》2018年第16期3036-3041,共6页Progress in Modern Biomedicine

基　　金：陕西省社会发展科技攻关项目(2016SF-131);国家自然科学基金项目(81773488;81373058;81370082)

摘　　要：目的:了解内蒙古赤峰市敖汉旗地区乙型肝炎病毒(HBV)基因型、血清型的分布特征。方法:收集2014年在赤峰市敖汉旗医院住院治疗的慢性乙型肝炎患者,采集血清标本,巢式PCR方法扩增HBV S区,测序后应用Mega6.0构建系统发育树确定该地区乙型肝炎病毒主要基因型、血清型,并分析HBV S基因a决定簇变异情况。结果:在72例HBV患者中,B基因12例(16.7%),C基因型40例(55.65%),D基因型20例(27.8%)。不同性别患者的基因型分布差异有统计学意义(P<0.01)。血清亚型分析结果显示:adw2血清亚型15例(20.8%)、adrq+血清亚型37例(51.4%)、ayw2血清亚型20例(27.8%)。HBV S基因"a"抗原决定簇突变率为34.7%。结论:内蒙古赤峰市敖汉旗地区HBV基因型以C型为主,其次是D型和B型,血清型主要为adrq+,有34.7%患者携带HBV S基因a决定簇变异株。Objective： To determine the distribution of hepatitis B virus（HBV）genotypes and its characteristics in Aohan area of Chifeng city,Inner Mongolia. Methods： The respondents contain the chronic hepatitis B patients of the Aohan hospital in 2014. Using the method of nested PCR amplification HBV gene sequences and phylogenetic tree was constructed to determine the main genotypes and serotype of hepatitis B. HBV S gene mutations were analyzed through multiple sequence alignment with sequences of HBV prevalence strains from China in Genbank. Results： We found 3 genotypes in 72 chronic hepatitis B patients： B（16.7%）,C（55.65%）,and D（27.8%）.There were significant differences in genotype distribution among patients with different gender（P〈0.01）. The proportion of adw2 was20.8%,adrq＋ was 51.4% and ayw2 was 27.8%. The mutation rate of a antigen determinant was 34.7%. Conclusions： The major genotypes of HBV prevalent among chronic hepatitis B patients in Aohan area of Inner Mongolia was C. And adrq＋ was the most predominant serotype. 34.7% of the patients were testified carrying HBV S gene a determinant cluster variant strains.

关 键 词：乙肝病毒 基因型 内蒙古 

分 类 号：R512.62[医药卫生—内科学] R181.2[医药卫生—临床医学]