黄芪破壁饮片的DNA条形码鉴别与黄酮类成分HPLC指纹图谱研究  

Study on DNA Barcode Identification and HPLC Fingerprint of Flavonoids in Ultrafine Granular Power of Radix Astragali

作　　者：王艳 彭丽华[2] 郑夏生 成金乐[2] Wang Yan;Peng Lihua;Zheng Xiasheng;Cheng Jinle(Research Center of Chinese Herbal Resource Science and Engineering,Guangzhou University of Chinese Medicine;Key Laboratory of Chinese Medicinal Resource from Lingnan,Ministry of Education（Guangzhou University of Chinese Medicine）,Joint Laboratory of National Engineering Research Center for the Pharmaceutics of Traditional Chinese Medicines,Guangzhou 510006,Chin;2.Key Laboratory of Cell-broken Decoction Pieces Technology and Application of State Administration of Traditional Chinese Medicine,ZEUS Pharmaceutical Group,Zhongshan 528437,China;3.Guangzhou University of Chinese Medicine,Guangzhou 510006,China)

机构地区：[1]广州中医药大学中药资源科学与工程研究中心,岭南中药资源教育部重点实验室(广州中医药大学),国家中成药工程技术研究中心南药研发实验室,广州510006 [2]国家中医药管理局中药破壁饮片技术与应用重点研究室,中山市中智药业集团有限公司,中山528437 [3]广州中医药大学,广州510006

出　　处：《世界科学技术-中医药现代化》2018年第5期789-797,共9页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：广东省科学技术厅产学研合作项目(2014B090905015)：广东省中智药业集团中药破壁饮片院士工作站,负责人：成金乐

摘　　要：目的:利用DNA条形码技术对黄芪破壁饮片进行物种鉴定,再建立其黄酮类成分HPLC指纹图谱,为其质量控制和鉴定提供依据。方法:提取15批样品的DNA进行序列分析和物种鉴定。物种鉴定后采用高效液相色谱法,色谱柱:Agilent ZORBAX SB-Aq C18(250 mm×4.6 mm,5μm);流动相:乙腈-0.15%甲酸溶液梯度洗脱;流速0.6 m L/min;检测波长254 nm;柱温25℃;结果:经DNA条形码鉴定,实验所用的15批样品均被鉴定为蒙古黄芪Astragalus membranaceus(Fisch.)Bge.var.mongholicus(Bge.)Hsiao。在此基础上建立了15批样品的HPLC指纹图谱,标定11个共有峰,15批样品的相似度在0.9-1.0之间。结论:DNA条形码和指纹图谱两种方法可对黄芪破壁饮片进行物种鉴定和全面反映黄芪破壁饮片中的成分信息,可用于黄芪破壁饮片的真伪鉴定和批间一致性评价。Objective： To identify species of Ultrafine Granular Powder of Radix astragali by DNA barcode, and to establish HPLC fingerprinting of Flavonoids, so as to provide basis for quality control and identification. Methods： The DNA of 15 batches of samples was extracted, and then the sequences and species were identified. After then the HPLC method was used. Chromatography conditions were Agilent ZORBAX SB-Aq C18（250 mm×4.6 mm, 5 μm） column with a gradient elution composed of acetonitrile-aqueous solution containing 0.15% formic acid, the flow rate was 0.6 m L/min and the column temperature was 25 ℃, While the detection wavelength was set at 254 nm. Results： By DNA barcode identifying, the 15 batches of samples used in this experiment were successfully identified as Astragalus membranaceus（Fisch.） var. mongholicus（Bge.） Hsiao. On this basis, HPLC fingerprinting of 15 batches of samples was established. A total of 11 common peaks were demarcated. Similarity of the fingerprinting spectrums of 15 batches of samples were between 0.9-1.0. Conclusion： The two methods of DNA barcode and HPLC fingerprinting were used to identify and fully reflect the composition information of Radix Astragali. It provides verification and consistency evaluation for Ultrafine Granular Powder of Radix Astragali.

关 键 词：黄芪破壁饮片 DNA条形码 HPLC指纹图谱 

分 类 号：R284.1[医药卫生—中药学]