二甲双胍及有氧运动对肥胖大鼠骨骼肌脂联素和葡萄糖转运体4的影响  

Effects of metformin and exercise on adiponectin and GLUT4 in skeletal muscles of obese rats

作　　者：童俊露 王佑民[2,3] 邓大同[3] TONG Junlu;WANG Youmin;DENG Datong(Taikang Xianlin Drum-tower Hospital,Nanjing,Jiangsu 210046,China;Institute of Endocrinology and metabolic diseases of Anhui Medical University,Hefei,Anhui 230022,China;Department of Endocrinology,The First Affiliated Hospital of Anhui Medical University,Hefei,Anhui 230022,China)

机构地区：[1]泰康仙林鼓楼医院,江苏南京210046 [2]安徽医科大学内分泌与代谢病研究所,安徽合肥230022 [3]安徽医科大学第一附属医院内分泌科,安徽合肥230022

出　　处：《安徽医药》2018年第10期1873-1876,2067,共5页Anhui Medical and Pharmaceutical Journal

摘　　要：目的探讨二甲双胍及有氧运动对高脂饲料喂养的肥胖大鼠骨骼肌中脂联素(APN)和葡萄糖转运体4(GLUT4)的影响,以及APN和GLUT4之间的关系。方法 4周龄50只雄性SD大鼠采用随机数字表法分为普通对照组(NC组)和高脂饮食组(HD组),NC组予普通饲料饲养,HD组高脂饲料喂养。12周后,HD组采用随机数字表法分为:二甲双胍组(MT组)、游泳运动组(SE组)和高脂肥胖组(HF组)。SE组大鼠每日游泳运动,MT组给予二甲双胍(每日200 mg·kg^(-1))灌胃,共6周,空腹取血测相关代谢指标,分离骨骼肌组织测APN和GLUT4的表达。结果 HF组大鼠体质量(BW)、三酰甘油(TG)、总胆固醇(TCH)、空腹血糖(FBG)、空腹胰岛素(FINS)水平、HOMA-IR指数均明显高于NC组(P<0.05),骨骼肌APN和GLUT4的表达较NC组明显下调(P<0.05)。运动、二甲双胍干预后能降低体质量(P<0.05),降低血糖、血脂(P<0.05),改善胰岛素抵抗(P<0.05)。运动干预同时上调骨骼肌中APN和GLUT4的表达(P<0.05),二甲双胍干预后骨骼肌中GLUT4的表达明显上调(P<0.05),APN表达变化不明显(P>0.05)。结论有氧运动可上调骨骼肌组织中APN及GLUT4的表达,二甲双胍可上调骨骼肌中GLUT4的表达,但对APN的表达无明显改善作用。Objective To investigate the effeCTs of metformin and aerobic exercise on adiponeCTin（ APN） and glucose transporter 4（ GLUT4） in skeletal muscle of obese rats fed with high fat diet,and the relationship between APN and GLUT4. Methods Fifty male Sprague-Dawley（ SD） rats aged 4 weeks were randomly assigned into normal control group（ group NC） and high fat diet group（ groupHD）. Group NC was given normal feeding,while groupHD was fed with high-fat diet. After 12 weeks,groupHD was assigned into metformin group（ group MT）,swimming group（ group SE） and high fat group（ groupHF）. Rats of group EC were daily swimming exercise,group MT received gavage of metformin（ 200 mg·kg^（-1）） for 6 weeks,fasting blood was taken to measure the metabolic indexes,the expression of APN and GLUT4 were determined by the separation of skeletal muscle tissue. Results The body weight（ BW）,blood lipids（ TG）,total cholesterol（ TCH）,fasting blood glucose（ FBG）,fasting insulin（ FINS） level and HOMA in rats of high fat diet（ groupHF）were significantly higher than those in group NC（ P〈0. 05）,the expression of APN and GLUT4 in skeletal muscle were significantly lower than that in group NC（ P〈0. 05）. Both exercise and metformin can significantly reduce the body weight,blood glucose and blood lipid,and improve insulin resistance（ all P〈0. 05）. Swimming exercise intervention can significantly upregulate the expression of APN and GLUT4 in skeletal muscle（ P〈0. 05）. The expression of GLUT4 in skeletal muscle was significantly up-regulated by metformin（ P〈0. 05）,and the expression of APN was not significantly changed（ P〉0. 05）. Conclusions Aerobic aCTivity can up-regulate the expression of APN and GLUT4 in skeletal muscle tissue. Metformin can up-regulate the expression of GLUT4 in skeletal muscle,but it has no obvious improvement on the expression of APN.

关 键 词：二甲双胍 脂联素 葡萄糖转运体4型 肥胖症 运动 大鼠 Sprague-Dawley 

分 类 号：R965[医药卫生—药理学]