未动员外周血造血干细胞采集的临床效果评价  

The clinical evaluation on the collection of peripheral blood stem cell without mobilization

作　　者：郑伟萍[1] 方琦[1] 陆紫敏[1] 刘承重 李雪晨[1] ZHENG Weiping;FANG Qi;LU Zirnin;LIU Chengzhong;LI Xuechen(Department of Blood Transfusion,the Affiliated Tongji Hospital of Shanghai Tongji University,Shanghai 200065,China)

机构地区：[1]同济大学附属同济医院输血科,上海200065

出　　处：《检验医学与临床》2018年第18期2690-2693,共4页Laboratory Medicine and Clinic

基　　金：上海市卫生局科研课题资助项目(2010046);上海市公共卫生重点学科建设计划资助项目(12GWZX0202)

摘　　要：目的探讨未动员外周血造血干细胞(PBSC)采集影响因素及采集效果。方法应用MCS+血细胞分离机对该院130例未动员采集者进行PBSC采集,获得单个核细胞(MNC)、白细胞(WBC)、CD34+细胞数分别与采集前体质量指数(BMI)、三酰甘油(TG)、载脂蛋白(apo)B、红细胞比容(Hct)、血小板(PLT)、WBC、血红蛋白(Hb)、MNC等指标,以及采集时循环数、循环血量等因素进行相关性分析;同时对其中50岁以下的2组(健康组与疾病组)的PBSC采集效果进行比较。结果所有采集者均采集成功:(1)其外周血中是存在并能够采集到一定数量的MNC、CD34+细胞的。(2)50岁以下采集者比较:健康组采集物中MNC、WBC计数均高于疾病组,差异有统计学意义(P<0.05);但2组采集物CD34+细胞数比较差异无统计学意义(P>0.05)。(3)采集物的MNC总量和CD34+细胞数量与采集前自身的apoB、PLT、WBC、Hb、TG均无明显相关性。采集前的外周血MNC、BMI、Hct及采集时循环血量、循环数对采集效果有相关影响,其中外周血MNC水平与采集物MNC水平呈正相关。结论未作动员的采集者其外周血可安全采集到一定浓度的、并能够满足临床治疗需要的PBSC。采集者自身PBSC采集效果与采集前MNC、BMI、Hct水平及采集时循环血量、循环数有关联,其中采集前MNC数量可预测采集者PBSC采集效果。Objective To investigate the possible factors that will affect the collection of peripheral blood stem cells without mobilization.Methods The autologous peripheral blood stem cells（PBSC）from 130 cases（without using mobilization with granulocyte colony stimulating factor（G-CSF））were collected by MCS＋blood cell separator.We investigated the correlation between the number of PBSC [collected by mononuclear cells（MNC）,white blood cell（WBC）and CD34＋,respectively]and the some possible factors[including body mass index（BMI）,triglyceride（TG）,apolipoprotein（apo）B,red blood cell hematocrit（Hct）,platelet（PLT）count,WBC count,hemoglobin（Hb）,MNC count,the number of cycles and the amount of blood circulation）].We also compared two groups（healthy vs.disease;under 50 years of age）of the efficiency of the PBSC collection.Results Our results showed that the peripheral blood were collected successfully from all 130 cases.In addition,we were able to collect a certain number of MNC,CD34＋cells.By comparing two groups of collectors（under 50 years of age）：the number of MNC and WBC of the health group were significantly higher than the ones of disease group（P〈0.05）.However,there was no significant difference（P〉0.05）in the number of CD34＋between two groups.There was no significant correlation in the number of MNC,CD34＋cells from peripheral blood stem cell and before collected in peripheral blood of apoB,PLT,WBC,Hb,and TG.In addition,there is a significant correlation in the efficiency of the collection from peripheral blood stem cell and before collected in peripheral blood of MNC,BMI,Hct and the number of cycles and the amount of blood circulation when being collected.The MNC concentration before and after collected was positively correlated.Conclusion PBSC can be collected from peripheral blood of collectors（the collectors of whom has not been mobilized）,and the concentration of it can meet the clinical needs.The correlation was observed between selfcollecte

关 键 词：肿瘤 外周血造血干细胞 采集效果 

分 类 号：R446.11[医药卫生—诊断学]