广泛耐药鲍曼不动杆菌的体外联合抑菌分析  

Analysis of combined antibacterial of extensively drug-resistant Acinetobacter baumannii in vitro

作　　者：马冬梅 陶庆春 齐宏伟 MA Dongmei;TAO Qingchun;QI Hongwei(Department of Clinical Laboratory,Beijing University of Chinese Medicine Third Affiliated Hospital,Beijing 100029,China)

机构地区：[1]北京中医药大学第三附属医院检验科,北京100029

出　　处：《中国医药导报》2018年第25期148-151,共4页China Medical Herald

基　　金：北京中医药大学自主选题项目(2016-JYB-JSMS-083)

摘　　要：目的研究双黄连和米诺环素、哌拉西林/他唑巴坦和头孢哌酮/舒巴坦联合应用对广泛耐药鲍曼不动杆菌的联合抑菌效果。方法收集2016年10月~2017年6月从北京中医药大学第三附属医院(以下简称"我院")临床标本分离出的30株广泛耐药鲍曼不动杆菌,应用肉汤稀释法测定双黄连与米诺环素、哌拉西林/他唑巴坦、头孢哌酮/舒巴坦的最低抑菌浓度(MIC),计算部分抑菌浓度(FIC)指数,判定中西药联合抑菌效果。结果双黄连和米诺环素联用FIC指数:0.5~1占6.7%、>1~2占63.3%、>2占30.0%;双黄连和哌拉西林/他唑巴坦联用FIC指数:0.5~1占16.7%、>1~2占83.3%;双黄连和头孢哌酮/舒巴坦联用FIC指数:≤0.5占23.3%、0.5~1占73.3%、>1~2占3.3%。结论双黄连联合米诺环素和哌拉西林/他唑巴坦对广泛耐药鲍曼不动杆菌多数表现为无关作用和拮抗作用,体外联合抑菌作用较差;而双黄连与头孢哌酮/舒巴坦联合应用体外抑菌作用较好。Objective To study antibacterial effect of Shuanghuanglian combined with minocycline and Piperacillin/Taz-obactam and Cefoperazone/Sulbactam on extensively drug-resistant Acinetobacter baumannii in vitro. Methods A total of 30 strains extensively drug-resistant Acinetobacter baumannii were collected from clinical specimens from October 2016 to June 2017 in Beijing University of Chinese Medicine Third Affiliated Hospital. The minimum inhibitory concentration（MIC） of Shuanghuanglian and minocycline, Piperacillin/Tazobactam, Cefoperazone/Sulbactam（Cefoperazone/Sulbactam） in 30 strains extensively drug-resistant Acinetobacter baumannii was tested by the broth dilution method, and the partial inhibitory concentration（FIC） index was calculated, and the combined bacteriostasis effect of Chinese and Western medicine was determined. Results The FIC index of Shuanghuanglian combined with minocycline： 0.5-1 accounted for 6.7%,〉 1-2 accounted for 63.3%, and 〉2 accounted for 30.0%; the FIC index of Shuanghuanglian combined with Piperacillin/Tazobactam： 0.5-1 accounted for 16.7%, 〉1-2 accounted for 83.3%; the FIC index of Shuanghuanglian combined with Cefoperazone/Sulbactam： ≤ 0.5 accounted for 23.3%, 0.5-1 accounted for 73.3%,〉 1-2 accounted for 3.3%. Conclusion Shuanghuanglian combined with minocycline and Piperacillin/Tazobactam has a non-independent and antagonistic effect on the extensively drug-resistant Acinetobacter baumannii,and the combined antibacterial effect in vitro is poor. The combined antibacterial effect of Shuanghuanglian combined with Cefoperazone/Sulbactam is better.

关 键 词：广泛耐药鲍曼不动杆菌 部分抑菌浓度 双黄连粉针剂 抑菌效果 

分 类 号：R446.5[医药卫生—诊断学]