Plackett-Burman与Box-Behnken试验设计优化苦豆子中氧化苦参碱提取工艺  

Extraction Technology Optimization of Oxymatrine from Sophora Alopecuroids L. by Plackett-Burman and Box-Behnken Experimental Design

作　　者：关亮俊 高荣凯[1] 任海东 肖顺丽 王敏[1] 张宏桂[1] 

机构地区：[1]北京中医药大学中药学院,北京102488

出　　处：《中国药师》2018年第9期1509-1513,共5页China Pharmacist

基　　金：国家自然科学基金项目(编号:31070328)

摘　　要：目的:优选苦豆子中氧化苦参碱的最佳提取工艺。方法:通过单因素试验和Plackett-Burman试验设计确定各因素的最佳水平并筛选出关键因素,采用Box-Behnken响应面法优选氧化苦参碱的最佳提取工艺。结果:氧化苦参碱的最佳提取工艺为:料液比1∶25,提取时间60 min,浸提温度60℃,乙醇浓度60%,提取3次,氧化苦参碱的提取率可达80.83%。结论:利用该方法优化出的苦豆子氧化苦参碱的提取工艺稳定可靠,可为氧化苦参碱的开发利用提供参考。Objective： To optimize the extraction process of oxymatrine from Sophora alopecuroids L.. Methods： The single factor extraction tests and Plackett-Burman experiment were employed to seleet the optimum levels and key factors, and then, the extraction process of oxymatrine was optimized by Box-Behnken experiment. Results： The optimized parameters were as follows ： the ratio of mate- rial to liquid was 1 ： 25, the extraction time was 60 min, the extraetion temperature was 60~C, the ethanol coneentration was 60%, and the extraction frequency was three times. Under the above conditions, the extraction efficiency reached 80. 83%. Conclusion： The optimized parameters from the experiments are stable and accurate, which can be used for the development and utilization of oxymatrine.

关 键 词：苦豆子 氧化苦参碱 提取工艺 PLACKETT-BURMAN设计 Box-Behnken响应面法 

分 类 号：R284.2[医药卫生—中药学]