HPLC法测定米诺地尔微乳中米诺地尔的含量  

Determination of Minoxidil in Minoxidil Microemulsion by HPLC

作　　者：冯佳玉 纪宏宇[1,2,3] 张诚诚 吴琳华 

机构地区：[1]哈尔滨医科大学附属第二医院药学部,哈尔滨150086 [2]哈尔滨医科大学药学院 [3]黑龙江省高校重点实验室

出　　处：《中国药师》2018年第9期1689-1692,共4页China Pharmacist

摘　　要：目的:建立高效液相色谱法测定米诺地尔微乳中米诺地尔的含量。方法:色谱柱:Diamonsil C_18柱(200 mm×4.6 mm,5μm),流动相:甲醇-水-冰醋酸-十二烷基硫酸钠(70∶30∶0.03∶0.045)(v/v/v/w),流速:1.0 ml·min^(-1),检测波长:280 nm,进样量:10μl,柱温:30℃。结果:米诺地尔在5.00~100.00μg·ml^(-1)线性关系良好(r=0.999 7),平均回收率为99.12%,RSD为1.60%(n=9)。结论:该方法简便,准确,灵敏,重复性好,专属性强,可用于该制剂的含量测定。Objective： To establish an HPLC method for the determination of minoxidil in minoxidil microeraulsion. Methods： A Diamonsil C18 column （ 200 mm × 4.6 mm, 5 μm ） was used with the mobile phase of methanol-water-glacial acetic acid-SDS （ 70 ： 30 ： 0.03 ： 0.045 ） （ v/v/v/u,）, the deteetion wavelength was 280 nra and the sample size was 10 μl. The flow rate was 1.0 ml ·min ^- 1 The column temperature was 30℃. Results： The calibration curve of minoxidil was linear within the concentration range of 5.0-100.0 μg · ml - 1 （ r = 0.999 7 ）. The average recovery was 99.12% （ RSD = 1. 60% , n = 9 ）. Condusion ： The method is accurate, reliable, simple and rapid, which is applicable in the determination of minoxidil in minoxidil micrrooemulsian.

关 键 词：微乳 高效液相色谱法 米诺地尔 含量测定 

分 类 号：R927.2[医药卫生—药学]