眼点拟微绿球藻养殖过程中细菌污染的治理方法  

Treatment Method of Bacterial Contamination in the Process of Nannochloropsis oculata Cultivation

作　　者：耿金峰 赵鄢鹏 冯倩 蔡忠贞 王慧岭 张晋阳 王冰 刘敏胜 白雪梅 GENG Jin-feng;ZHAO Yan-peng;FENG Qian(Xin＇ao Technology Development Co.,Ltd.,State Key Laboratory of Coal-Based Low Carbon Energy,Langfang,Hebei 065001)

机构地区：[1]新奥科技发展有限公司,煤基低碳能源国家重点实验室,河北廊坊065001

出　　处：《安徽农业科学》2018年第26期63-67,76,共6页Journal of Anhui Agricultural Sciences

基　　金：中美清洁能源联合研究中心项目(CERC)“微藻固碳产业化技术研究与示范”(2016YFE0102500-09)

摘　　要：[目的]以进入中国饲料原料目录的眼点拟微绿球藻为研究对象,研究其在户外自然培养过程受到细菌污染时的污染治理方法及方法的应用。[方法]在硫酸庆大霉素、四环素、青霉素、红霉素和氯霉素为代表的5大类抗生素中筛选最佳的抗生素种类,并对藻液中抗生素残留进行氧化、紫外方法单独使用或结合使用等处理方法的研究。[结果]综合抗生素对藻细胞本身的影响和对细菌的治理效果结果,硫酸庆大霉素治理细菌效果最优,其次为四环素;红霉素对细菌无明显抑制作用;青霉素和氯霉素对藻细胞生长有明显的抑制作用。去除残留庆大霉素的有效方法为:在不含藻细胞的水体中可单独通入2 h的臭氧或加入100 mg/L高浓度有效氯溶液的方法可将硫酸庆大霉素去除95%以上,该方法可用作养殖清液或废液中硫酸庆大霉素的处理方法;在藻液中可使用15 mg/L的有效氯+紫外照射0.5 h的组合方法联合处理形式效果佳,藻液中的硫酸庆大霉素去除率在90%以上;藻细胞生长仅在初期受到轻微抑制作用,但细胞性状很快得到恢复,总体藻细胞的性状基本未受到明显的影响。[结论]针对户外眼点拟微绿球藻养殖过程中细菌污染可以使用硫酸庆大霉素进行治理,并可利用15 mg/L有效氯+紫外照射0.5 h将残留在藻液中的庆大霉素较好地去除,上述2种方法的结合使用可以保证户外养殖的持续进行,为眼点拟微绿球藻的规模化稳定养殖提供一种污染治理技术支撑,有利于该藻株的产业化进程。[Objective]Taking the Nannochloropsis oculata as the research object in the catalogue of Chinese feed ingredients, the pollution control method when the outdoor natural culture process was contaminated by bacteria and the application of method was studied.[Method]The best antibiotic species among the five major classes of antibiotics represented by gentamicin sulfate, tetracycline, penicillin, erythromycin and chloramphenicol were screened,and the treatment of antibiotic residues in algae liquid was oxidized, and the ultraviolet method was used alone or in combination.[Result]An effective way to remove residual gentamicin was：In the water body without algae cells, the gentamicin sulfate could be removed by more than 95% by adding 2 hours of ozone alone or by adding 100 mg/L of high concentration of available chlorine solution,the method could be used as a treatment method for gentamicin sulfate in a culture supernatant or waste liquid.In the algae solution, 15 mg/L of effective chlorine ＋ UV irradiation for 0.5 h could be combined to form a good effect, and the removal rate of gentamicin sulfate in the algae solution was above 90%.Algae cell growth was only slightly inhibited at the beginning, but the cell traits were quickly restored, and the overall algal cell traits were not significantly affected.[Conclusion]For the bacterial contamination in the process outdoor cultivation of Nannochloropsis oculata, it can be treated with gentamicin sulfate, and the gentamicin remaining in the algae solution can be better removed by using 15 mg/L available chlorine ＋ UV irradiation for 0.5 h.The combination of the above two methods can ensure the continuous cultivation of outdoor culture, and provide a pollution control technical support for the large-scale stable culture of Nannochloropsis oculata, which is beneficial to the industrialization process of the algae strain.

关 键 词：眼点拟微绿球藻 细菌污染 抗生素 治理方法 

分 类 号：S946[农业科学—水产养殖]