金线莲不同繁殖途径比较  

Comparison on Different Rapid Propagation Pathways of Anoectochilus roxburghii

作　　者：郑枫[1] 黎倩芸 叶志聪 曾宋君[1] 吴坤林[1] ZHENG Feng;LI Qian-yun;YE Zhi-cong(South China Botanical Garden,Chinese Academy of Sciences,Guangzhou,Guangdong 510650;Guangzhou Ganruntang Biological Co.,Ltd.,Guangzhou,Guangdong 510900)

机构地区：[1]中国科学院华南植物园,广东广州510650 [2]广州甘润堂生物科技有限公司,广东广州510900

出　　处：《安徽农业科学》2018年第26期94-97,共4页Journal of Anhui Agricultural Sciences

基　　金：广东省产学研项目(2013B091500045);广东省星火计划项目(2013B020405007);广东省林业科技创新项目(2018KJCX031)

摘　　要：[目的]筛选出高效的金线莲种苗工厂化繁殖技术体系。[方法]分别以野生金线莲种子进行非共生萌发和以茎段为外植体进行无性克隆2种途径繁育种苗。[结果]2种繁殖途径在诱导初始条件、增殖方式、增殖系数、继代周期、根的诱导分化及移栽等方面均存在差异。[结论]金线莲以无性克隆繁殖途径为宜,建立的繁育体系具有经济、高效、稳定等优点,该研究可为规模化育苗提供理论和技术参考。[Objective]The industrial production technology system of Anoectochilus roxburghii seedling was screened out.[Method]By comparing the different propagation methods of A. roxburghii , we screened out the efficient seedling breeding methods. In this study, non-symbiotic germination of wild A. roxburghii seeds and asexual cloning with stem segments as explants were carried out.[Result]There were differences between the two pathways, such as induced initial conditions, proliferation ways, propagation coefficient, subculture cycle, root induction and seedling transplanting. [Conclusion] According to the comparative test, it’s more suitable for constructing the tissue culture system with asexual cloning, which is more economical, more efficient and steady, and it will provide material and technical reference for large-scale seedling production.

关 键 词：金线莲 繁殖途径 组织培养 

分 类 号：S567.23[农业科学—中草药栽培]