化学发光微粒子免疫分析法在梅毒血清学检测中的应用  

The application value of chemiluminescent microparticle immunoassay in serological detection of syphilis

作　　者：张伦军[1] 丁晓琳[1] 朱安友[1] ZHANG Lun-jun;DING Xiao-lin;ZHU An-you(Department of Laboratory Medicine,The First Affiliated Hospital of Bengbu Medical College,Bengbu Anhui 233004,China)

机构地区：[1]蚌埠医学院第一附属医院检验科,安徽蚌埠233004

出　　处：《蚌埠医学院学报》2018年第8期1057-1059,共3页Journal of Bengbu Medical College

基　　金：安徽省教育厅重点项目(KJ2015A219)

摘　　要：目的:探讨化学发光微粒子免疫分析法(CMIA)在梅毒螺旋体抗体血清学检测中的应用价值。方法:采用酶联免疫吸附试验(ELISA)法对临床标本作梅毒螺旋体特异性抗体筛查,对筛选出的86例阳性标本进一步采用CMIA和梅毒螺旋体明胶颗粒凝集试验(TPPA)检测,分析比较检测结果,并将CMIA测定值与甲苯胺红不加血清热试验(TRUST)检测结果进行比较。结果:86例ELISA试验阳性标本中,经TPPA确证试验,阳性84例,阴性2例,ELISA试验符合率为97.67%;而CMIA和TPPA检测符合率为100%。TRUST假阴性率较高,且随着CMIA的检测值增高,TRUST阳性率明显增加(P<0.01)。结论:ELISA法筛查梅毒存在一定的假阳性,CMIA可以作为梅毒螺旋体特异性抗体筛查阳性标本的确证试验,TRUST可用于疗效观察。Objective： To investigate the application value of chemiluminescent microparticle immunoassay（ CMIA） in serological detection of syphilis. Methods： The special antibody of treponema pallidum in clinical specimens were screened using enzyme-linked immunosorbent assay（ ELISA）,and the 86 positive samples were detected by CMIA and treponema pallidum particle agglutination assay（ TPPA）. The detection result was analyzed,and the value of CMIA was compared with the tolulized red unheated serum test（ TRUST）.Results： Among 84 positive samples detected by ELISA,84 positive samples and 2 negative samples were identified using the TPPA detection. The coincidence rate of ELISA test was 97. 67%,and the coincidence rate of CMIA and TPPA was 100%. The false negative rate of TRUST was higher,and the positive rate of TRUST increased significantly with the increasing of detection value of CMIA（ P 0. 01）. Conclusions： There is certain extent false positive rate in the syphilis screened by ELISA,CMIA can be the confirmation experiment in the screening of syphilis specific antibody. TRUST is suitable for the observation of curative effect.

关 键 词：梅毒 化学发光微粒子免疫分析法 特异性抗体检测试验 

分 类 号：R759.1[医药卫生—皮肤病学与性病学] R446.6[医药卫生—临床医学]