五味子乙素通过Traf6/TAK1信号通路介导人胃癌细胞凋亡  

Effects of schisandrin B on human gastric cancer cell apoptosis via Traf6/TAK1 signaling pathway

作　　者：王金桥 邓银芝 WANG Jin-qiao;DENG Yin-zhi(Department of Gastroenterology,Central Hospital of En-shiAutonomous Prefecture,Enshi 445000,China)

机构地区：[1]恩施土家族苗族自治州中心医院消化内科,湖北恩施445000

出　　处：《实用药物与临床》2018年第9期970-973,共4页Practical Pharmacy and Clinical Remedies

摘　　要：目的研究五味子乙素介导人胃癌HGC27细胞凋亡发生的作用及对Traf6/TAK1信号通路的影响。方法不同浓度的五味子乙素(12、24、48μg/ml)干预细胞后,采用CCK8法检测HGC27细胞增殖情况,流式细胞术分析五味子乙素诱导HGC27细胞发生凋亡的情况,免疫印迹法分析五味子乙素对HGC27细胞中Bax、Bcl-2、Traf6、TAK1及p-TAK1蛋白表达量的影响。结果与对照组比较,五味子乙素能显著抑制HGC27细胞增殖,诱导细胞凋亡;诱导促凋亡蛋白Bax表达,抑制抗凋亡蛋白Bcl-2蛋白表达;显著抑制Traf6、p-TAK1蛋白表达,且呈浓度依赖性,差异均有统计学意义(P <0. 05)。结论五味子乙素能明显抑制HGC27细胞增殖、诱导凋亡,其作用机制可能与抑制Traf6/TAK1信号通路活化有关。Objective To study the impacts of schisandrin B on human gastric cancer cell （ HGC27） apoptosis and on the activity of Traf6/TAK1 signaling pathway. Methods Following incubation with different concentrations of schisandrin B （12,24 and 48 μg/ml）, CCK8 assay was used to detect the HGC27 proliferation, the apoptosis rates were investigated by flow cytometry, and the expression levels of Bax, Bcl-2 , Traf6 , TAK1 and p-TAK1 were deter-mined using Western blot method. Results Compared with control gro u p , the proliferation inhibition rates and apopto-sis rates of HGC27 cells were significantly increased ; the expression level of Bax was significantly increased and the ex-pression level of Bcl-2 was greatly decreased ; the expression levels of Traf6 and p-TAK1 were greatly decreased in a concentration-dependent manner, the differences being statistically significant （ P 〈0.05） . Conclusion Schisandrin B can inhibit the proliferation of HGC27 cells and stimulate the apoptosis. Traf6/TAK1 signaling pathway may be in-volved in the inhibitory effect of schisandrin B on the tumor cells.

关 键 词：五味子乙素 人胃癌细胞 增殖 凋亡 

分 类 号：R285[医药卫生—中药学]