壮骨止痛方对去势大鼠Wnt/β-catenin信号通路的影响  

Effect of Zhuanggu-Zhitong Prescription on Wnt/β-catenin Signaling Pathway of Ovariectomized Rats with Osteoporosis

作　　者：蹇秀林 刘湘琳 梁琼[1] 刘平安[1] 张国民[1] Jian Xiulin;Liu Xianglin;Liang Qiong;Liu Pingan;Zhang Guomin(Hunan Univercity of Chinese Medicine,Changsha,410208,China)

机构地区：[1]湖南中医药大学,湖南长沙410208

出　　处：《成都中医药大学学报》2018年第3期26-28,33,127,共5页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：湖南省大学生研究性学习和创新性实验计划项目(2015217;2015220;2016284;2016281;2017281);湖南省中医药科研计划重点项目(201612);湖南省教育厅科学研究重点项目(16A162);湖南省自然科学基金面上项目(2018JJ2297)

摘　　要：目的:观察壮骨止痛方对去势大鼠骨组织Wnt/β-catenin信号通路的影响,探讨其治疗骨质疏松的机制。方法:60只3月龄SD雌性大鼠,按体重随机分为假手术组、空白组、模型组、壮骨止痛方组,15只/组。模型组、壮骨止痛方组采用双侧去卵巢建立绝经后骨质疏松大鼠模型,假手术组切除卵巢周围相应质量脂肪。术后5 d开始药物干预,连续13 w。取股骨,观察病理形态及Wnt7b、TCF3、GSK3β蛋白的表达。结果:与模型组相比,壮骨止痛方组骨小梁密度及梁髓比均提高。与模型组相比,壮骨止痛方组骨组织中Wnt7b、TCF3蛋白表达增高,GSK3β蛋白表达降低,差异具有统计学意义(P <0. 05)。结论:壮骨止痛方可通过上调去势大鼠骨组织中Wnt7b、TCF3蛋白表达,下调GSK3β蛋白表达来调控Wnt/β-catenin信号通路。To explore the effect of Zhuanggu-Zhitong Prescription on Wnt/p-catenin Signaling Pathway of Ovariectomized Rats with Osteoporosis, and to investigate its mechanism in anti- osteoporosis. Methods： 60 three-month old SD female rats, according to the weight, were randomly divided into sham-operated group, blank group, model group and Zhuanggu-Zhitong Prescription group, 15 rats in each group. The model group ’ s and Zhuanggu-Zhitong Prescription group ’ s rats were ovariecto- mized, and the sham-operated group’s rats were excised equal weight fat. After 5 days of the operation, rats started to receive drug therapy. 13 weeks later, the bone tissue pathological change and the protein expression of Wnt7b, TCF3, GSK3p were investigated. Results： Compared with the model group, Zhuanggu-Zhitong Prescription group increased the trabecular bone density and the ratio of bone trabecular to marrow cavity. Compared with the model group, the protein expression of Wnt7b, TCF3 were increased in Zhuanggu-Zhitong Prescription group, and the protein expression of GSK3p was decreased, the difference had statistical signifi-cance （P 〈 0. 05 ）. Conclusion： Zhuanggu-Zhitong Prescription can regulate the Wnt/pcatenin Signaling Pathway by rasing the expressions of protein Wnt7b, TCF3 and reducing the expression of protein GSK3p.

关 键 词：壮骨止痛方 绝经后骨质疏松症 WNT/Β-CATENIN信号通路 

分 类 号：R285.5[医药卫生—中药学] R588[医药卫生—中医学]