四种污染源气体颗粒物对大鼠血清肌红蛋白和脑钠肽的影响  

The effects of four different pollutants on the levels of serum myoglobin and brain natriuretic peptide in rats

作　　者：齐铁雄[1] 石琳[1] 郑彤[1] 包敏[1] 刘世华[2] 刘艳[1] QI Tiexiong;SHI Lin;ZHENG Tong;BAO Min;LIU Shihua;LIU Yan(Department of Cardiovascular Medicine,The Affiliated Children Hospital of the;Nephrology Department Beijing 100020,China Beijing 100012,China)

机构地区：[1]首都儿科研究所附属儿童医院心血管内科,北京100020 [2]航空总医院肾内科,北京100012

出　　处：《西部医学》2018年第9期1252-1256,共5页Medical Journal of West China

基　　金：首都儿科研究所方向性引导基金(FX-2016-08)

摘　　要：目的观察四种不同污染源气体颗粒物对大鼠血清肌红蛋白和脑钠肽水平的影响。方法将70只成年SD大鼠随机分为对照组10只、汽车尾气组15只、煤烟组15只、燃烧秸秆组15只和自然空气组15只;按1mg/ml的相同的浓度气管内分别滴注汽车尾气、煤烟、燃烧秸秆、自然空气颗粒物悬液每天1次,一次0.1ml,连滴10天后,采用化学发光法动态检测5组的血清肌红蛋白(Mb)和脑钠肽(BNP)水平;计算HM/BM;CCK-8法检测大鼠心肌细胞的存活率,并观察心肌细胞的病理变化。结果各研究组大鼠血清Mb的水平均有所升高,均在一定时间内达到峰值。血清中BNP水平也存在此类波动变化,BNP达到峰值的时间及恢复正常水平的时间比Mb水平晚,但升高的幅度大。汽车尾气组、煤烟组和自然空气组大鼠HM/BM明显升高,出现了不同程度的心肌细胞浸润、水肿、变性、坏死、纤维化;心肌细胞存活率明显下降;秸秆组大鼠HM/BM变化不明显,心肌病理出现了轻度的心肌细胞水肿、变性,HM/BM无明显升高,心肌细胞存活率下降不明显。结论高浓度的汽车尾气、煤烟、燃烧秸秆、自然空气颗粒均可以引起大鼠的心肌损害。检测大鼠血清中肌红蛋白(Mb)和脑钠肽(BNP)的水平对判断污染物对大鼠心肌损害程度具有重要意义。Objective To observe the effects of four different pollutants on the levels of serum myoglobin and brain natriuretic peptide in rats.Methods 70 adult SD rats were randomly divided into the control group（10 rats）,the vehicle exhaust group（15 rats）,the coal smoke group（15 rats）,the burning straw group（15 rats）,and the natural air group（15 rats）.According to the same concentration of 1 mg/ml,the vehicle exhaust,soot,burning straw,natural air particulate suspension（1 time,0.1 ml,10 days）were respectively injected into the trachea.The levels of serum myoglobin（Mb）and brain natriuretic peptide（BNP）in 5 groups were detected by chemiluminescence.The survival rate of myocardial cells was detected by cck-8,and the pathological changes of myocardium were observed.Results The levels of Mb in the serum of rats in the study group were all increased,which reached the peak within a certain time.There were also such fluctuations in the BNP level in the serum,and the peak time of the BNP and the recovery time tonormal levelwerelater than thoseof Mb,but the increase was large.In the automobile exhaust group,the soot group and the natural air group,HM/BM was significantly elevated,with varying degrees of myocardial infiltration,edema,degeneration,necrosis and fibrosis.The survival rate of myocardial cells decreased significantly.The change of HM/BM in the straw group was not obvious,and the myocardial pathology showed mild myocardial edema,degeneration,no significant increase in HM/BM,and the survival rate of myocardial cells was not obvious.Conclusion High concentration of automobile exhaust,soot,burning straw and natural air particles can cause myocardial damage in rats.The levels of myoglobin（Mb）and brain natriuretic peptide（BNP）in rat serum were found to be significant in determining the degree of myocardial damage in rats.

关 键 词：污染源 颗粒物 肌红蛋白 脑钠肽 

分 类 号：R54[医药卫生—心血管疾病]