苦参碱预处理对大鼠脑缺血再灌注损伤外周血Th1/Th2细胞因子影响作用  

Effects of Matrine Preconditioning on Th1/Th2 Cytokines in Peripheral Blood of Rats with Cerebral Ischemia-reperfusion Injury

作　　者：肖旭[1] 石文达[1] 才春华[1] 黄伟[1] 胡久丽[1] 李代晓[1] 赵艳武 XIAO Xu;SHI Wenda;CAI Chunhua(The Affiliated Hospital of Chengde Medical College,Hebei Chengde 067000,China)

机构地区：[1]承德医学院附属医院药剂科,河北承德067000

出　　处：《河北医学》2018年第9期1416-1420,共5页Hebei Medicine

基　　金：河北省承德市科学技术研究与发展计划项目;(编号:20122128)

摘　　要：目的:探讨通过苦参碱预处理后,对大鼠脑缺血再灌注损伤外周血Th1/Th2细胞亚群及其活化的细胞因子的影响作用。方法:建立大鼠全脑缺血再灌注损伤模型,将50只SD大鼠随机分为5组,分别为假手术对照组、手术模型组、苦参碱(4mg/kg,8mg/kg,16mg/kg)预处理组。手术前48h,假手术对照组与手术模型组大鼠分别腹腔注射等量的0.9%氯化钠注射液,药物预处理组腹腔注射相应剂量的苦参碱溶液,每日2次,每次2m L,共给药2日。于脑缺血再灌注后4h,8h,16h,24h进行股动脉取血流式细胞仪检测T细胞亚群;分离血清,采用ELISA法检测细胞因子(IL-1β、TNF-α、IL-4、IL-10)。结果:苦参碱各预处理后可明显降低外周血CD4^+T细胞水平降低(P<0.05或P<0.01),升高CD8^+T细胞水平(P<0.05或P<0.01),降低Th1细胞活化的促炎细胞因子(TNF-α、IL-1β)的水平(P<0.01或P<0.05),升高Th2细胞活化的抗炎细胞因子(IL-4、IL-10)的水平(P<0.01或P<0.05)。结论:苦参碱通过调节Th1/Th2细胞亚群,调整后Th1/Th2细胞活化的细胞因子水平,减轻炎症反应,达到脑神经缺血再灌注损伤的保护作用。从而起到对大鼠脑缺血再灌注损伤的神经细胞保护作用。Objective： To investigate the effects of Matrine on Th1/Th2 subsets and activated cytokines in peripheral blood of rats with cerebral ischemia reperfusion injury. Methods： 50 SD rats were randomly divided into 5 groups,namely sham operation group,model group,matrine pretreatment group（ 4 mg/kg,8 mg/kg,16 mg/kg）. 48 hours before operation,rats in both sham operation group and model group were gave saline by intraperitoneal injection,other drug pretreatment group were gaven matrine injection,respectively,bid for 2 days. Cerebral ischemia-reperfusion injury model in rats were established. 4 h,8 h,16 h,24 h later,T cell subsets in femoral artery blood were detected by flow cytometry. Then the serum was separated and the cytokines（ IL-1β,TNF-α,IL-4,and IL-10） were detected by ELISA. Results： The matrine pretreatment can significantly reduce the level of CD4＋T cells in peripheral blood（ P〈0.05 or P〈0.01）,increase the level of CD8＋T cells（ P〈0.05 or P〈0.01）,reduce the level of proinflammatory cytokines TNF-α and IL-1β in the activated Th1 cell（ P〈0.05 or P〈0.01）. Increase the level of anti-inflammatory cytokines IL-4 and IL-10 in the activated Th2 cells（ P〈0.05 or P〈0.01）. Conclusion： Matrine can play a neuroprotective role on cerebral ischemia reperfusion injury in rats by regulating Th1/Th2 cell subsets,adjusting cytokines in activated Th1/Th2 cell and inhibiting inflammation reaction.

关 键 词：苦参碱 脑缺血-再灌注损伤 TH1/TH2细胞亚群 细胞因子 

分 类 号：R285.5[医药卫生—中药学]