GA20-氧化酶基因转化豆科牧草百脉根的研究  被引量：13

Transformation of Leguminous forage Lotus corniculatus by Agrobacterium mediated GA 20oxidase gene</em> <p class='article-op'> <span id="bdshare" class="bdshare_t bds_tools get-codes-bdshare baidufx"><span><span class="bds_more" style="display: block; width: 62px; height: 29px; background: url(/images/share.gif) no-repeat!important; padding: 0;"></span></span></span> </p> <p class="article-source"> <a href ='/article/read.aspx?id=6762470' class='btnTitle btnIsView' target='_blank'><img alt='在线阅读' src='/images/btn-read.png'/></a><a class='btnTitle btnIsView' href="javascript:showdown('Rxjhdh9keiP5MTRC+mV5t/S59QczaorPtWiNwmO3Irs=')"><img alt="下载全文" src="/images/btn-down.png" /></a> <input id="hiddowninfo" type="hidden" value="Rxjhdh9keiP5MTRC+mV5t/S59QczaorPtWiNwmO3Irs="/> </p> <div class="collect" style="display: none"> <h5>我的收藏</h5> <div class="cnt"> <p> 名 称：<input type="text" name="collTitle" id="collSumTitle" /> </p> <p> 注 释：<input type="text" name="collRemark" id="collSumRemark" /> </p> <p style="text-align: center;"> <a href="javascript:void(0)" onclick="g_collectSumOk();"> <img width="65" height="17" src="/images_new/collect_ok.png" alt="完成" /></a> <a href="javascript:void(0)" onclick="g_collectSumCancel();"> <img width="65" height="17" src="/images_new/collect_no.png" alt="取消" /></a> </p> </div> </div> </div> <form name="wxTransfer" method="post"> <input type="hidden" name="lid" value="" /> <input type="hidden" name="sip" value="" /> <input type="hidden" name="date" value="" /> <input type="hidden" name="co" value="" /> <input type="hidden" name="Title_C" value="" /> <input type="hidden" name="Writers" value="" /> <input type="hidden" name="Name_C" value="" /> <input type="hidden" name="Num" value="" /> <input type="hidden" name="Years" value="" /> <input type="hidden" name="Volumn" value="" /> <input type="hidden" name="GCH" value="" /> <input type="hidden" name="Vol" value="" /> <input type="hidden" name="BookID" value="" /> <input type="hidden" name="SubjectNum" value="" /> <input type="hidden" name="SpecialNum" value="" /> <input type="hidden" name="mirrorName" value="" /> <input type="hidden" name="UserID" value="" /> <input type="hidden" name="UserName" value="" /> <input type="hidden" name="sourceID" value="" /> </form> <script type="text/javascript" src="/scripts/transfer.min.js"></script> <div class="article-detail"> <p class='author'><strong>作　　者：</strong><a href='/writer/article.aspx?id=100000007410951' target='_blank' ><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=A%3d%5b%e5%bc%a0%e6%8c%af%e9%9c%9e%5d' title='张振霞[1]'>张振霞[1]</a> <a href='/writer/article.aspx?id=100000012537258' target='_blank' ><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=A%3d%5b%e5%82%a8%e6%88%90%e6%89%8d%5d' title='储成才[2]'>储成才[2]</a> <a href='/writer/article.aspx?id=100000000590520' target='_blank' ><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=A%3d%5b%e7%ac%a6%e4%b9%89%e5%9d%a4%5d' title='符义坤[1]'>符义坤[1]</a> <em> </em></p><p class='organ'><strong>机构地区：</strong><a href='/organ/article.aspx?id=13647&subid=&showname=&searchtype=' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=S%3d%e7%94%98%e8%82%83%e5%86%9c%e4%b8%9a%e5%a4%a7%e5%ad%a6%e8%8d%89%e4%b8%9a%e5%ad%a6%e9%99%a2' title='[1]甘肃农业大学草业学院,兰州730070'>[1]甘肃农业大学草业学院,兰州730070</a> <a href='/organ/article.aspx?id=18947&subid=&showname=&searchtype=' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=S%3d%e4%b8%ad%e5%9b%bd%e7%a7%91%e5%ad%a6%e9%99%a2%e9%81%97%e4%bc%a0%e5%8f%91%e8%82%b2%e7%a0%94%e7%a9%b6%e6%89%80' title='[2]中国科学院遗传发育研究所,北京100101'>[2]中国科学院遗传发育研究所,北京100101</a> <em> </em></p><p class='from'><strong>出　　处：</strong><a href='/journal/summary.aspx?gch=97784X' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=J%3d%5b%e8%8d%89%e4%b8%9a%e5%ad%a6%e6%8a%a5%5d' title='草业学报'>《草业学报》</a>2002年第3期97-100,共4页<em>Acta Prataculturae Sinica</em></p><p class='fund'><strong>基　　金：</strong>.NULL.</p><p class='abstrack'><strong>摘　　要：</strong>以豆科植物百脉根子叶为转化受体,通过根癌农杆菌介导方法将外源目的基因GA20-氧化酶(GA20-oxidase)和NPTII基因导入,经筛选分化、再生,得到具有卡那霉素抗性的转基因植物。在卡那霉素筛选浓度的选择中,25mg/L是百脉根较为适宜的筛选浓度。头孢霉素对农杆菌的抑制作用好于羧卞霉素。转基因植物移栽大田后生长良好。PCR检测证明外源目的基因已整合到百脉根基因组中。<em><Abstrcat> Transgenic plant of Lotus corniculatus L. was obtained by Agrobacteriummediated transformation, utilizing a binary vector containing GA 20oxidase gene and Kanamycin resistance gene NPTII. Transgenic plants grew well after transferring to greenhouse. PCR analysis confirmed that GA 20oxidase gene and NPTII gene were integrated into the Lotus corniculatus genome.</em></p><p class='subject'><strong>关 键 词：</strong><a href='/zk/search.aspx?key=K%3d%5bGA20-%e6%b0%a7%e5%8c%96%e9%85%b6%5d' title='GA20-氧化酶'>GA20-氧化酶</a> <a href='/subject/article.aspx?id=5631276' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e5%9f%ba%e5%9b%a0%e8%bd%ac%e5%8c%96%5d' title='基因转化'>基因转化</a> <a href='/subject/article.aspx?id=430901' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e8%b1%86%e7%a7%91%e7%89%a7%e8%8d%89%5d' title='豆科牧草'>豆科牧草</a> <a href='/subject/article.aspx?id=7368449' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e7%99%be%e8%84%89%e6%a0%b9%5d' title='百脉根'>百脉根</a> <a href='/subject/article.aspx?id=6018567' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e6%a0%b9%e7%99%8c%e5%86%9c%e6%9d%86%e8%8f%8c%5d' title='根癌农杆菌'>根癌农杆菌</a> <a href='/subject/article.aspx?id=1529571' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e6%a4%8d%e7%89%a9%e5%86%8d%e7%94%9f%5d' title='植物再生'>植物再生</a> </p><p class='class'><strong>分 类 号：</strong><a href='/zk/search.aspx?key=C%3dS541.6' title='S541.6'>S541.6</a>[<a href = '/domain/article.aspx?id=534' >农业科学—作物学</a>] </p> </div> <div id="content_wzxg" name="wzxg" class="article-graph"> <object width="695" height="250" classid="clsid:D27CDB6E-AE6D-11cf-96B8-444553540000" id="FlashID"> <param value="../flash/relation.swf" name="movie"> <param value="high" name="quality"> <param value="opaque" name="wmode"> <param value="6.0.65.0" name="swfversion"> <param value="dataUrl=/ajax/articlerelativegraph.ashx?id=6762470" name="flashVars"> <embed width="695" height="250" src="../flash/relation.swf" type="application/x-shockwave-flash" quality="high" wmode="opaque" flashvars="dataUrl=/ajax/articlerelativegraph.ashx?id=6762470"> </embed> 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