血必净对急性百草枯中毒大鼠肾脏细胞凋亡及其调控因子Bcl-2、Bax、Caspase-3表达的影响  

The effects of Xuebijing injection on apoptosis and the expression of regulatory factors including Bcl-2,Bax and Caspase-3 in the renal cells of rats with acute paraquat poisoning

作　　者：赵艳霞[1] 付赟[2] 王鑫[3] ZHAO Yanxia;FU Yun;WANG Xin(Department of Broken Stone,Central Hospital of Handan City,Hebei,Handan 056001,China)

机构地区：[1]河北省邯郸市中心医院体外碎石室,056001 [2]河北省邯郸市中心医院肾内一科,056001 [3]河北省邯郸市中心医院急诊科,056001

出　　处：《河北医药》2018年第19期2889-2894,共6页Hebei Medical Journal

摘　　要：目的通过观察急性百草枯中毒大鼠肾组织中Bcl-2、Bax、Caspase-3的表达及和TUNEL检测细胞凋亡率的变化,探索血必净对百草枯中毒的治疗作用。方法将126只Wister大鼠采用随机数字表法分为对照组、染毒组、治疗组。每组在1 d、3 d、7 d、14 d、21 d、28 d、35 d各取6只,用水合氯醛腹腔注射进行麻醉,留取肾组织标本,行免疫组化检测凋亡蛋白Bcl-2、Bax、Caspase-3的表达及TUNEL检测凋亡细胞率。结果在染毒组,大鼠肾组织中Bax、Caspase-3含量及TUNEL细胞凋亡率明显高于对照组(P<0.05),Bcl-2低于对照组(P<0.05)。经血必净治疗后,Bax、Caspase-3含量及TUNEL细胞凋亡率与中毒组比较,明显降低(P<0.05),Bcl-2在各时间点高于中毒组(P<0.05);与对照组比较,Bax、Caspase-3含量及TUNEL细胞凋亡率升高,Bcl-2含量各时段仍较低,差异有统计学意义(P<0.05)。结论 Bcl-2、Bax、Caspase-3在百草枯所致大鼠肾损伤中起重要作用;血必净增强了Bcl-2的表达,抑制了染毒大鼠肾组织中Bax、Caspase-3的含量,降低细胞凋亡率,减轻染毒大鼠肾组织损伤。Objective To investigate the therapeutic effects of Xuebijing on paraquat poisoning through observing the expressions of Bcl-2,Bax and Caspase-3 and the changes of apoptosis rate detected by TUNEL in kidney of acute paraquat poisoning rats. Methods A total of 126 Wister rats were randomly divided into control group（ n = 42）,contamination group（ n = 42）,treatment group（ n = 42）. At 1 d,3 d,7 d,14 d,21 d,28 d and 35 d after treatment,the 6 rats in each group were sacrificed by chloral hydrate intraperitoneal injection anesthesia. The renal tissue samples were collected and the expression of Bcl-2,Bax and Caspase-3 were detected by immunohistochemistry as well as cell apoptosis were detected by TUNEL. Results The Bcl-2,Bax and Caspase-3 content and apoptosis rate in contamination group were significantly higher than those in control group（ P〈0. 05）,however the expression levels of Bcl-2 in contamination group were significantly lower than those in control group（ P〈0. 05）. After Xuebijing treatment,the contents of Bax,Caspase-3 and the rate of apoptosis in treatment group were significantly lower than those in contamination group（ P〈0. 05）,and the expression levels of Bcl-2 in treatment group were significantly higher than those in contamination group in every time point（ P〈0. 05）. The contents of Bax,Caspase-3 and the rate of apoptosis in treatment group were significantly increased,as compared with those in control group（ P〈0. 05）. Moreover the content of Bcl-2 in treatment group was significantly lower than that in control group（ P〈0. 05）.Conclusion The Bcl-2,Bax and Caspase-3 proteins play an important role in renal injury of rats caused by paraquat poisoning. Xuebijing can enhance the expression of Bcl-2,inhibit the contents of Bax and Caspase-3 in renal tissue,decrease apoptosis rate so as to relieve the damage of renal tissue of rats with poisoning.

关 键 词：百草枯 肾损伤 BCL-2/BAX Caspase-3 血必净 

分 类 号：R595.4[医药卫生—内科学]