农杆菌介导茄子叶转基因体系的建立  

Establishment of Agrobacterium tumefaciens-mediated genetic transformation system for eggplant cotyledons

作　　者：李想 张峻 周露 盛力立 陈火英[1] LI Xiang;ZHANG Jun;ZHOU Lu;SHENG Li li;CHEN Huo ying(School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China;Minhang District Agricultural Technology Service Center,Shanghai 201109,China;Shengeng Agriculture Development Co.Ltd,Shanghai 201808,China)

机构地区：[1]上海交通大学农业与生物学院,上海200240 [2]闵行区农业技术服务中心,上海201109 [3]申耕农业发展有限公司,上海201808

出　　处：《上海交通大学学报（农业科学版）》2018年第4期1-6,共6页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：国家自然科学基金(31471870)

摘　　要：本文选择3份茄核心种质,研究Hyg B浓度、农杆菌浓度(OD600)、侵染时间、共培养时间对茄遗传转化效果的影响。同时,选择7份核心种质进行转化研究,以确定茄遗传转化的最佳受体。结果表明,筛选阳性植株的最适Hyg B浓度为50mg/L;菌液浓度(OD_(600))0.6,侵染外植体15min和共培养4d时,抗性愈伤百分率最高;获得的Hyg B抗性植株,经PCR检测及GUS组织化学鉴定,确认外源基因GUS已成功整合到茄基因组中;不同茄遗传型转化效果差异较大,最高转化率可达到10%。Three eggplant core germplasm resources were selected to study the effects of hygromycin B concentration,Agrobacterium concentration（OD600）,infection time and co-culture time on the genetic transformation.At the same time,seven core germplasm resources were used to confirm the best receptor for eggplant genetic transformation.The results showed that the most suitable hygromycin B concentration for screening positive plants was 50 mg/L.The percentage of resistant calluses was the highest with the combination of 0.6 OD600 for Agrobacterium,15 minutes of explant infection duration and 4 days of coculture duration.PCR and GUS analysis of hygromycin B-resistant plants showed that the GUSgene was successfully integrated into the eggplant genome,and there was great variation in transformation rate among different genotypes,the highest transformation rate being 10%.

关 键 词：茄 农杆菌 转基因 遗传型筛选 转基因植株 

分 类 号：S641.1[农业科学—蔬菜学]