基于核酸适配体的阳离子聚合物聚集纳米金比色法检测水中睾酮  

Colorimetric assay for testosterone detection based on cationic polymer aggregating gold nanoparticles in water

作　　者：邱建敏[1] 倪璇[1] 周晓彤 张通 徐鲁荣[1] 王鲁梅[1] QIUJian-min;NI Xuan;ZHOUXiao-tong;ZHANG Tong;XULu-tong;WANG Lu-mei(Key Laboratory of Urban Agriculture,Ministry of Agriculture,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China)

机构地区：[1]上海交通大学农业与生物学院农业部都市农业(南方)重点实验室,上海200240

出　　处：《上海交通大学学报（农业科学版）》2018年第4期7-13,共7页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：上海市浦江人才计划(16PJD026);国家重点研发计划项目(2017YFD0800704);教育部第50批留学回国人员科研启动基金(王鲁梅);国家自然科学基金面上项目(20977062)

摘　　要：利用核酸适配体对睾酮特异性识别功能以及阳离子聚合物PDDA(poly dimethyl diallyl ammonium chloride,PDDA)聚集纳米金特性,建立了一种高灵敏度、高特异性测定睾酮的定量方法。在最优实验条件[10 mmol/L PB缓冲溶液(pH 7.4),孵育温度25℃,6nmol/L PDDA,6nmol/L睾酮核酸适配体]下,体系吸光度比值A_(650)/A_(520)与睾酮浓度C呈良好的线性关系,决定系数R2为0.995。该方法可检测睾酮浓度范围为2.27nmol/L^1.8μmol/L,最低检测限为2.27nmol/L,将本方法应用于检测自来水睾酮含量,加标回收率为99.81%~105.04%。本方法具有检测范围广、成本低、操作简便、检测灵敏等优点,具有良好实际应用前景。A highly sensitive and specific strategy for testosterone detection was developed based on the recognition by nucleic acid aptamer and aggregation of gold nanoparticles by poly dimethyl diallyl ammonium chloride（PDDA）.Under the optimized condition of this detection system（10 mmol/L PBS（pH 7.4）,reaction temperature 25 ℃,6 nmol/L PDDA,6 nmol/L testosterone-specific aptamer）,the absorbance ratio A_（650）/A_（520） has a good linear relationship with the concentration of testosterone,and RSquare is 0.995.The limit of detection is 2.27 nmol/L in this method,with a detection range from 2.27 nmol/L to 1.8μmol/L.Applying the method to detect testosterone content in tap water,the recovery rate of testosterone is between 99.81 % and 105.04 %.This method has many advantages,such as wide range of detection,low cost,simple operation and sensitive detection,and has a good prospect of practical application.

关 键 词：睾酮 核酸适配体 阳离子聚合物 纳米金 检测 

分 类 号：O657.3[理学—分析化学] X836[理学—化学]