敲降钙调磷酸酶B同源蛋白2表达导致静脉血管内皮细胞增殖迁移抑制和细胞周期停滞的研究  

CHP2 knockdown inhibits the proliferation and migration of vascular endothelial cells and inducesc ell cycle arrest

作　　者：张剑[1] 龚昆梅[1] 赵泉[1] 龙亚新[1] 肖乐[1] 郭晓东[1] ZHANG Jian;GONG Kun-mei;ZHAO Quan;LONG Ya-xin;XIAO Le;GUO Xiao-dong(First Department of General Surgery,The First People＇s Hospital of Yunnan Province,The Affiliated Hospital of Kunming University of Science and Technology,Kunming 650000,China)

机构地区：[1]云南省第一人民医院昆明理工大学附属医院普外一科,昆明650000

出　　处：《中国血管外科杂志（电子版）》2018年第2期133-137,共5页Chinese Journal of Vascular Surgery(Electronic Version)

基　　金：云南省科技厅-昆明医科大学应用基础研究联合专项基金(2017FE467)

摘　　要：目的探索钙调磷酸酶B同源蛋白2(CHP2)表达降低对静脉血管内皮细胞增殖和迁移的影响及其可能机制。方法通过使用靶向CHP2的si RNA降低CHP2在人脐静脉血管内皮细胞(HUVEC)中的表达,CCK-8法和流式细胞仪检测CHP2表达降低对细胞增殖和细胞周期的影响,划痕实验和Transwell实验检测CHP2对细胞迁移的作用,Western blot法检测CHP2敲降对活化T细胞核因子(NFAT)入核的影响。结果 CHP2表达降低有效抑制HUVEC细胞增殖,与G0/G1细胞周期停滞相关,CHP2敲降也抑制了HUVEC细胞迁移,机制研究表明CHP2表达降低对细胞增殖和迁移的影响可能与其抑制NFAT入核相关。结论 CHP2的表达降低抑制了静脉血管内皮细胞增殖和迁移,这可能导致静脉血管缺损修复的抑制。Objective To explore the effect of reduced expression of calcineurin B homologous protein 2 （CliP2） on proliferation and migration of venous vascular endothelial cells and corresponding mechanism. Methods CliP2 expression in human umbilical vein endothelial cells （HUVEC） was decreased by using small interfering RNA （siRNA） targeting CliP2. CCK-8 assay and flow cytometry were adopted to detect the impact of CHP2 knockdown on cell proliferation and cell cycle of HUVEC. The effect of decreased CliP2 expression on cell migration was evaluated by Scratch test and a Transwell test. In addition, the influence of CliP2 knockdown on nuclear factor of activated T cells （NFAT） nuclear translocation was detected by western blotting assay. Results CHP2 knockdown effectively inhibited the proliferation of HUVEC cells and which was related to the cell cycle arrest at G0/G1 phase. In addition, CHP2 knockdown also inhibited the migration of HUVEC cells. The mechanism study shows that the effect of CliP2 downregulation on cell proliferation and migration may be related to the inhibition of NFAT nuclear translocation. Conclusion The decreased expression of CliP2 inhibits the proliferation and migration of venous endothelial cells, which may lead to repair inhibition of damaged venous vascular.

关 键 词：深静脉血栓形成 静脉血管 内皮细胞 增殖 迁移 钙调磷酸酶B同源蛋白2 

分 类 号：R543.6[医药卫生—心血管疾病]