金针菇菌糠纤维素的制备条件优化  

Optimization of Preparation Conditions for Spent Mushroom Substrate Cellulose of Flammulina velutiper(Fr.) Sing

作　　者：乔洁[1,2,3] 解春艳 侯晓强[1,2,3] 李丛胜 QIAO Jie;XIE Chlzn-yan,;HOU Xiao-qiang;LI Cong-shengI(Langfang Normal University, Langfang 065000, China;Edible and Medicinal Fungi Research and Development Center of Hebei Universities, Langfang 065000, China;Edible Fungi Key Laboratory of Langfang City, Langfang 065000, China)

机构地区：[1]廊坊师范学院,河北廊坊065000 [2]河北省高校食药用菌资源开发应用技术研发中心,河北廊坊065000 [3]廊坊市食用菌技术重点实验室,河北廊坊065000

出　　处：《廊坊师范学院学报（自然科学版）》2018年第3期69-73,共5页Journal of Langfang Normal University(Natural Science Edition)

基　　金：河北省高校食药用菌资源开发应用技术研发中心(YF201411);河北省大学生创新创业训练计划(201510100015);廊坊师范学院微生物学重点学科项目(2015001)

摘　　要：采用碱水解法制备金针菇菌糠纤维素。以纤维素含量和木质素含量为指标,通过单因素和正交试验研究料液比、提取时间、提取温度、氢氧化钠浓度对纤维素含量和木质素含量的影响。结果表明:各因素对金针菇菌糠纤维素提取的影响大小为提取时间(B)>提取温度(C)>料液比(A)>氢氧化钠浓度(D)。金针菇菌糠纤维素的最佳制备条件为料液比1:15、提取时间60min、提取温度100℃、氢氧化钠浓度1.0mol·L-1,此条件下,经验证所得金针菇菌糠纤维素含量为67.57%,木质素含量为11.77%。Preparation of cellulose from spent mushroom substrate of Flammulina velutiper （Fr.） Sing by the method of alkaline hydrolysis was investigated in this study. The effects of solid-liquid ratio, extraction time, extraction temperature and NaOH concentration on the content of cellulose and lignin were studied by single factor and orthogonal experiment. Results indicated that these four factors influenced the cellulose extraction significantly, and the influence size order was extraction time〉 extraction temperature〉solid-liquid ratio〉NaOH concentration. The optimal condition was solid-liquid ratio 1：15, ex- traction time 60min, extraction temperature at 100 ℃, and NaOHconcentration 1.0 mol. L^-1, under this condition ,the highest cellulose content was obtained, which was 67.57% of cellulose.

关 键 词：金针菇菌糠 纤维素 木质素 优化 

分 类 号：S646.1[农业科学—蔬菜学]