应用肝素建立老龄双足小鼠退变性脊柱侧凸模型  

Heparin for establishment of degenerative scoliosis model in aging bipedal mice

作　　者：李洁 李娜 陈家瑜[3] 周艳辉 赵静 陆声[3] 麻伟青 徐永清[3] LI Jie;LI Na;CHEN Jia-yu;ZHOU Yan-hui;ZHAO Jing;LU Sheng;MA Wei-qing;XU Yong-qing(Department of Orthopedic Surgery;Department of Anesthesiology;Department of Health Care,Kunming General Hospital of PLA,Kunming,650032 China)

机构地区：[1]解放军昆明总医院保健科,云南昆明650032 [2]解放军昆明总医院麻醉科,云南昆明650032 [3]解放军昆明总医院附属骨科医院,云南昆明650032

出　　处：《中国矫形外科杂志》2018年第17期1605-1608,共4页Orthopedic Journal of China

基　　金：国家自然科学基金项目(编号:81701117;81400924);云南省应用基础研究计划项目(编号:2015FD073;2017FB112)

摘　　要：[目的]观察应用肝素致老龄双足直立雄性C57BL/6J小鼠脊柱侧凸的发生情况,为选择理想的退变性脊柱侧凸模型提供依据。[方法]采用无脊柱侧凸18个月龄雄性C57BL/6J老龄小鼠45只,随机分为三组,每组15只。对照组为老龄正常四足鼠,未经手术或药物处理;肝素组为老龄双足鼠皮下注射肝素;盐水组为老龄双足鼠皮下注射生理盐水。后两组手术截除双侧前肢制备为双足直立小鼠模型,于术后第8 d开始分别皮下注射肝素(1 IU/g)和等容量生理盐水,1次/d,持续4周。术后3个月进行前后位X线片拍摄评估三组小鼠脊柱侧凸发生情况,比较三组间脊柱侧凸发生率和侧凸严重程度。[结果]与对照组比较,肝素组脊柱侧凸发生率显著增高(P<0.01),盐水组脊柱侧凸发生率增高(P<0.05)。与盐水组比较,肝素组脊柱侧凸发生率显著增高,差异有统计学意义(P<0.01)。与对照组比较,肝素组和盐水组平均Cobb角显著增大,差异具有统计学意义(P<0.05);与盐水组比较,肝素组平均Cobb角显著增大,差异具有统计学意义(P<0.01)。[结论]肝素促进老龄双足小鼠退变性脊柱侧凸的发生,且脊柱侧凸程度严重,时间相对较短,为研究退变性脊柱侧凸的发病机制及治疗策略提供了良好的动物模型。[Objectives] To establish a degenerative scoliosis（DS） model in forelimb-amputated bipedal aging mice using heparin.[Methods] Forty-five C57 BL/6 J male mice aged 18 months without scoliosis were chosen and randomly divided into three groups with 15 animals in each group.Of them,mice in the control group remained normal quadrupedal ambulation without any surgery and drug given; while the animals in the heparin group received subcutaneously heparin（1 IU/g） injection daily and those in the saline group received subcutaneously normal saline for 4 weeks beginning at 8 days after bilateral forelimbs were amputated to create bipedal ambulation models.At 3 months after operation,the incidence of scoliosis and curve magnitude were compared among the 3 groups by anteroposterior X-rays.[Results] In term of the incidence of scoliosis,both the heparin group and the saline group were statistically significantly higher than the control group（P〈0.01 and P〈0.05）,while the heparin group proved statistically significantly higher than the saline group（P〈0.01）.Regarding to the curve magnitude,the heparin group and the saline group also had significantly greater Cobb angle than the control group（P〈0.05）,whereas the heparin group achieved significantly larger Cobb angle than the saline group（P〈0.01）.[Conclusions] The heparin does enhance occurrence of scoliosis in C57 BL/6 J male bipedal mice,with more severe deformity in a relative short term,which provides an animal for researches on pathogenesis and treatment of degenerative scoliosis.

关 键 词：退变性脊柱侧凸 肝素 双足直立雄鼠 

分 类 号：R682.3[医药卫生—骨科学]