12个新城疫病毒分离株的F基因和HN基因序列分析  

Sequence Analyses of F and HN Genes of 12 Newcastle Disease Virus Strains

作　　者：曹梦蕊 李荣旭 谭华龙 陈济铛 张溢珊 刘敏芳 陈建红 张济培 CAO Meng-rui;LI Rong-xu;TAN Hua-long;CHEN Ji-dang;ZHANG Yi-shan;LIU Min-fang;CHEN Jian-hong;ZHANG Ji-pei(College of Life Sciences and Engineering,Foshan University,Foshan,Guangdong,528231,China)

机构地区：[1]佛山科学技术学院生命科学与工程学院,广东佛山528231

出　　处：《动物医学进展》2018年第9期30-35,共6页Progress In Veterinary Medicine

基　　金：广东省科技计划项目(2015A020224044);广东省教育厅预防兽医学重点实验室项目(2014KTSPT037);广东省教育厅"创新强校工程"科研项目(校级;201508);广东省自然科学基金(2017A030310573);佛山市动物性食品安全监控研究平台(2014AG10022)

摘　　要：自1997年-2016年从广东佛山、肇庆、云浮等地疑似新城疫的家禽病料分离鉴定获得12株新城疫病毒,采用RT-PCR方法对所有分离株的F基因和HN基因进行扩增,产物经克隆并测序后与GenBank数据库中的参考毒株做遗传进化分析。12个分离株的F基因遗传进化分析结果显示,9株属于基因Ⅶ型,2株属于基因Ⅸ型,1株属于基因Ⅵ型。F基因同源性分析结果显示,12个毒株与目前疫苗株La Sota、B1、Mukteswar和Clone30的同源性在81.6%~91.5%之间,12个毒株间同源性的差异较大,9个Ⅶ型分离株之间的同源性在84.8%~99%之间;HN基因同源性分析结果显示,12个毒株与目前疫苗株La Sota、B1、Mukteswar和Clone30的同源性在80.9%~91.9%之间,12个毒株间同源性的差异较大,9个Ⅶ型分离株之间的同源性在84%~98.5%之间。氨基酸序列分析表明,12个分离株与NDV强毒株的氨基酸特征相符,有10个分离株的HN基因第514氨基酸残基出现Ⅰ→Ⅴ的变异,有9个分离株的F基因和HN基因的中和抗原位点出现变异。试验提示,当前广东部分地区的分离株与传统疫苗株之间存在抗原性差异,故应加强广东地区新城疫病毒的分子遗传监控。In this research, samples were collected from different poultry in Foshan, Zhaoqing, Yunfu, etc, Guangdong province from 1997 to 2016.The F genes and HN genes of 12 NDV isolates were amplified through RT-PCR.PCR products were cloned into 18-T vector and sequenced.The sequence results were an- alyzed by comparing with NDV reference strains published in GenBank.The genetic evolution analysis of F gene showed that 9 of 12 NDV isolates belong to genotype Ⅶ ,2 of 12 NDV isolates belong to genotype Ⅸ, I of 12 NDV isolates belongs to genotype Ⅵ.The aligment and analysis results showed that the F gene has similarities from 81.6% to 91.5% by comparing with those reference strains,such as La Sota,B1,Muk- teswar and Clone 30.The difference of similarity among 12 strains is larger.The similarities among 9 strains of type Ⅶ isolates are between 84.8% to 99%.The aligment and analysis results showed the HN gene has similarities from 80.9% to 91.9% by comparing with those reference strains,such as La Sota,B1,Muk- teswar and Clone 30.The difference of similarity among 12 strains is larger.The similarities among 9 strains of type Ⅶ isolates are between 84% to 98.5%.The analysis of sequences of amino acids showed that 12 i- solates were virulent strains of NDV.The 514th amino acid residues in the HN gene of 10 isolates showed I to V variation.And there are 9 isolates having the variation of the neutralizing antigen loci of the F and HN genes.The results suggest that there are antigenic differences between isolates and traditional vaccine strains in some areas of Guangdong. Therefore, molecular genetic monitoring of Newcastle disease virus strains in Guangdong area should be strengthened.

关 键 词：新城疫病毒 F基因 HN基因 序列分析 

分 类 号：S852.659.5[农业科学—基础兽医学] Q789[农业科学—兽医学]