抗鸡致病性大肠埃希菌复方中草药超微粉的急性与亚慢性毒性试验  

Acute and Subchronic Toxicity Tests of Compound Chinese Herbal Medicine Ultrafine Powder against Pathogetic E.coli in Chickens

作　　者：张召兴[1] 闫艳娟 李佩国[1] 李蕴玉[1] 贾青辉[1] 张香斋[1] ZHANG Zhao-xing;YAN Yah-juan;LI Pei-guo;LI Yun-yu;JIA Qing-hui;ZHANG Xiang-zhai(Hebei Key laboratory of Preventive Veterinary Medicine,Hebei Normal University of Science ﹠ Technology,Qinhuangdao,Hebei,066604,China)

机构地区：[1]河北科技师范学院河北省预防兽医学重点实验室,河北秦皇岛066604

出　　处：《动物医学进展》2018年第9期122-125,共4页Progress In Veterinary Medicine

基　　金：河北省现代农业产业技术体系蛋肉鸡产业创新团队项目(HBCT2018150206)

摘　　要：旨在评价抗鸡致病性大肠埃希菌(E.coli)复方中草药超微粉临床使用的安全性。在急性毒性试验中,分别按0.5、1.5、2.5、3.5、4.5、5.5、6.5、7.5g/(kg·d)给药,测定半数致死量(LD50);按照5、15、25g/(kg·d)给要测定最大耐受量;在亚慢性毒性试验中,在基础日粮添加20、40、80g/kg的复方中草药超微粉饲喂雏鸡至42日龄,在试验后的14d和28d,观察其临床表现和病理变化,测定体质量、肝肾功能生化指标。结果显示,急性毒性试验中未测出LD50,最大耐受量超过25g/(kg·d);亚慢性毒性试验中,试验后的14d,40g/kg和80g/kg添加组的体质量显著低于对照组(P<0.05),但在试验后的28d及停药后的14d,差异不显著(P>0.05);在试验后的14d和28d,20、40、80g/kg添加组血清中的GPT与GOT含量与对照组比较均不显著(P>0.05);40g/kg和80g/kg添加组血清中的BUN、UA含量均显著低于对照组(P<0.05)。复方中草药超微粉对雏鸡无急性毒性和亚慢性毒性,临床上使用是安全的。This study aimed at evaluating the safety of compound Chinese herbal medicine ultrafine powder in clinicl use.In the acute toxicity test, 0.5,1.5,2.5,3.5,4.5,5.5,6.5,7.5 g/（kg·d） were administered respectively,and the median lethal dose LD50 was measured; 5,15, and 25 g/（kg·d） were administered respectively,and the maximum tolerance was measured.In the subchronic toxicity test,we added 20 g/kg, 40 g/kg and 80 g/kg compound Chinese herbal medicine ultrafine powde to the basic ration of tested chickls until to 42 d, and on the 14 d and 28 d after the experiment,the clinical signs and histopathological lesions were observed,and the body mass,biochemical indexes of liver and kidney were detected.The re- sults showed that,in the acute toxicity test, LD50 was not detected, and the maximum tolerance limit was more than 25 g/（kg·d）,in the subcronic toxicity test,in the 14 d,the body weights of the 40 g/kg and 80 g/kg adding groups were significantly lower than that of control group （P〈0.05） ,but there was no sig- nificant difference in 28 d and 14 d after administration （P〉0.05） ; in the 14 d and 28 d serum GPT and GOT in 20 g/kg,40 g/kg and 80 g/kg groups were not significantly higher than that in the control group （P〉0.05）.BUN and UA of 40 g/kg and 80 g/kg groups were lower than that of control group（P〈0.05）. The ultrafine powder of compound Chinese herbal medicine has no acute toxicity and subchronic toxicity to chicks, and it is safe to use in clinical practice.

关 键 词：复方中草药超微粉 急性毒性试验 最大耐受试验 亚慢性毒性试验 雏鸡 

分 类 号：S853.75[农业科学—临床兽医学] S859.82[农业科学—兽医学]