逆灌注对大鼠肝移植后肾氧化应激损伤的影响  

Impact of retrograde reperfusion on renal oxidative stress injury after liver transplantation in rats

作　　者：程远[1] 邓桥 蔡秋程[3] 江艺[3] 吴爱平[4] Cheng Yuan;Deng Qiao;Cai Qiucheng;Jiang Yi;Wu Aiping(Department of Hepatobiliary Surgery,Fuzhou General Hospital ＆ Bengbu Medical College,Fuzhou 350025,Chin;Department of Hepatobiliary Surgery,Fuzhou General Hospital ＆ Fujian Medical Univer-sity,Fuzhou 350025,China;Department of Hepatobiliary Surgery,Fuzhou General Hospital,Fuzhou 350025,China;Department of Comparative Medicine,Fuzhou General Hospi-tal,Fuzhou 350025,China)

机构地区：[1]蚌埠医学院福总教学医院肝胆外科,福州350025 [2]福建医科大学福总教学医院肝胆外科,福州350025 [3]福州总医院肝胆外科,350025 [4]福州总医院比较医学科,350025

出　　处：《中华实验外科杂志》2018年第9期1667-1669,共3页Chinese Journal of Experimental Surgery

基　　金：福建省科技项目（2015Y0026）;福建省自然科学基金科技项目（2016J01585）

摘　　要：目的观察经下腔静脉逆灌注法对大鼠自体原位肝移植（OLT）后肾氧化应激的影响。方法将24只雄性SD大鼠随机分为假手术（Sham）组、经下腔静脉逆灌注（RETR）组和经门静脉灌注（IPR）组，每组8只。RETR组和IPR组大鼠建立自体OLT模型，其中RETR组采用经下腔静脉逆灌注法，IPR组采用经门静脉正灌注法。术后6h取双肾组织行光镜检查，并检测肾皮质丙二醛（MDA）含量、8．羟基脱氧鸟苷（8-OHdG）浓度、超氧化物歧化酶（SOD）活性及线粒体膜电位（A,m）水平。结果组织学检查示肾小管问质病变分级评分与分组间存在正相关关系（P=0．001），相关系数为0．628。RETR组MDA、8-OHdG[（21．79±0．95）nmol／mg·pro、（7．77±0．17）ng／m1]显著高于Sham组[（13．60±0．55）nmol／mg·pro、（6．29±0．09）ng／ml；P=0．000、0．001]，而显著低于IPR组[（24．96±0．68）nmol／mg·pro、（8．58±0．21）ng／ml；P=0．000、0．001]。RETR组SOD、△ψm[（1．46±0．05）U／mg·pro、4．42±0．14]显著低于Sham组[（2．46±0．07）U／mg·pro、6．05±0．32；P：0．000、0．001]，而显著高于IPR组[（1．07±0．04）U／mg·pro、3．77±0．08；P=0．000、0．001]。结论逆灌注可减轻大鼠自体原位肝移植后。肾氧化应激损伤，进而减轻肾缺血再灌注损伤。Objective To investigate the impact of retrograde reperfusion via vena cava on renal oxidative stress injury after autologous orthotopic liver transplantation （OLT） in rats. Methods 24 male Sprague Dawley （SD） rats were randomly divided into three groups, including sham operation （Sham） group, retrograde reperfusion via inferior vena cava （RETR） group and initial portal vein reperfusion （IPR） group, 8 rats in every group. Rats models of autologous OLT were established for RETR group and IPR group. Retrograde reperfusion via inferior vena cava was applied in RETR group, whereas IPR group used the initial portal vein reperfusion. Kidney samples in all rats were taken at six hours after operation for light microscopic examination ; renal cortex samples were homogenated to detect the content of malondialde- hyde （ MDA）, 8 - hydroxy - 2＇ - deoxyguanosine （ 8 - OHdG）, superoxide dismutase （SOD） and mito- chondrial membrane potential （△ψm）. Results Histological examination demonstrated a positive rank correlation relationship between the degree of tubular and interstitial damage with groups （ P = 0. 001 ）, and Spearman＇ s rank correlation coefficient was 0. 628. MDA and 8 -OHdG in RETR group [ （21.79 ± 0. 95） nmol/mg-pro, （7. 77±0. 17） ng/ml] were significantly higher than in Sham group [ （ 13.60±0. 55） nmol/mg.pro, （6. 29±0. 09） ng/ml; P =0. 000, 0. 001 ], while were significantly lower than in IPR group [ （24. 96 ± 0. 68） nmol/mg.pro, （8. 58 ±0. 21 ） ng/ml ; P = 0. 000, 0. 001 ]. SOD and △ψm in RETR group [ （ 1.46 ± 0. 05 ） U/mg- pro, 4. 42 ±0. 14 ] were significantly lower than in Sham group [ （2. 46±0. 07） U/mg＇pro, 6. 05 ±0. 32; P =0. 000, 0. 001], however were significantly higher than in IPR group [（1.07±0.04） U/mg-pro, 3.77±0.08; P=0.000, 0.001]. Conclusion Approach ofret-rograde reperfusion via inferior vena cava significantly reduces the renal oxidative stress injury and conse- quently protects kidney against is

关 键 词：逆灌注 SD大鼠 原位肝移植 肾损伤 氧化应激 

分 类 号：R657.3[医药卫生—外科学] R692[医药卫生—临床医学]