人脂肪干细胞PD—L1和Gal-9的表达及其影响因素  

Expression of PD-L1 and Gal-9 in human adipose-derived stem cells and its influencing factors

作　　者：周楷荐 郭澍[1] 李菲 乔莹[3] 梁国新[4] 张晓维 Zhou Kaijian;Guo Shu;Li Fei;Qiao Ying;Liang Guoxin;Zhang Xiaowei(Department of Plastic Surgery,First Hospital of China Medical University,Shenyang 110001,China)

机构地区：[1]中国医科大学附属第一医院整形外科,沈阳110001 [2]中国医科大学附属第一医院药学部,沈阳110001 [3]中国医科大学附属第一医院中心实验室,沈阳110001 [4]中国医科大学附属第一医院P3实验室,沈阳110001

出　　处：《中华整形外科杂志》2018年第8期661-668,共8页Chinese Journal of Plastic Surgery

基　　金：国家自然科学基金（51272286）;辽宁省自然科学基金（20102296）;国家卫生和计划生育委员会公益性行业科研专项（2015SQO0049）

摘　　要：目的 探讨人脂肪干细胞（adipose-derived stem cells,ADSCs）免疫共抑制分子PD-L1和Gal-9的表达及其影响因素.方法 培养并鉴定28例行吸脂术健康女性ADSCs,采用流式细胞术检测PD-L1和Gal-9表达,采用多因素线性回归分析年龄、身高体重指数（BMI）、脂肪采集部位以及干扰素-γ（interferon-γ,IFN-γ）对ADSCs中PD-L1和Gal-9表达水平的影响.结果 体外培养细胞符合ADSCs国际标准,且表达PD-L1和Gal-9.17例乳腺ADSCs中PD-L1和Gal-9的表达分别为37.24％±8.20％和4.41％ ±2.65％,显著高于11例腹部的28.80％±8.59％和2.51％±1.39％（P=0.018,P=0.039）.多因素线性回归分析显示,年龄和采集部位是影响PD-L1表达的独立危险因素（P=0.009,P=0.006）,年龄每增加1岁PD-L1表达降低0.385％,乳腺部位比腹部高8.58％.采集部位是影响Gal-9表达的独立危险因素（P=0.041）,乳腺部位比腹部高1.898％.BMI不是ADSCs表达PD-L1和Gal-9的影响因素.经20 ng/ml IFN-γ体外刺激48 h后,ADSCs的PD-L1阳性细胞百分比为49.850％±4.414％,显著高于刺激前（30.790％±3.602％,P=0.0036）;Gal-9阳性细胞百分比为5.084％±0.619％,显著高于刺激前（2.173％±0.267％,P=0.0004）.结论 人ADSCs表达PD-L1和Gal-9,且供者年龄小、采集乳腺部位及IFN-γ显著增加PD-L1和Gal-9表达.Objective To explore whether human adipose-derived stem cells （ADSCs） express PD-L1 and Gal-9 and its potential influence factors.Methods ADSCs isolated from 28 healthy female donors who underwent liposuction of the abdomen or breast tissue were cultured and characterized.The expression of PD-L1 and Gal-9 were detected using flow cytometry.The impact of donor age,body mass index （BMI）,donor site and interferon-γ （IFN-γ） on the expression of PD-L1 and Gal-9 was analyzed using multivariate linear regression analysis.Results The cultured cells fulfilled the criteria for defining MSCs according to the international standards and expressed PD-L1 and Gal-9.Breast-derived ADSCs had higher expression levels of PD-L1 （37.24% ± 8.20%） and Gal-9 （4.41% ± 2.65%） than those in abdomen-derived ADSCs （28.80% ± 8.59% and 2.51% ± 1.39%,respectively） （P =0.018,P =0.039）.Multivariate linear regression analysis showed that donor age and site were independent risk factors affecting PD-L1 expression （P =0.009,P =0.006）.The expression of PD-L1 decreased by 0.385% for every one year of age increase,and it was 8.58% higher in the breast-derived ADSCs than in the abdomen-derived ADSCs.Donor site was an independent risk factor for Gal-9 expression （P =0.041）.Gal-9 positive expression rate in the breast-derived ADSCs was 1.898% higher than that in the abdomen-derived ADSCs.BMI was not a risk factor affecting PD-L1 or Gal-9 expression on ADSCs.The expression of PD-L1 and Gal-9 on ADSCs were significantly upregulated after 48 hours stimulation with 20 ng/ml IFN-γin vitro.Conclusions Human ADSCs expressed PD-L1 and Gal-9.Donor age,site and IFN-γ treatment were independent risk factors affecting the expression of PD-L1 and Gal-9 on ADSCs.

关 键 词：脂肪干细胞 程序性细胞死亡配体-1 半乳糖凝集素-9 干扰素-Γ 

分 类 号：R622[医药卫生—整形外科]